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    NIH - Funded Training in Cell Signaling & Lung Pathobiology
    		 

    Al-Mehdi, Abu-Bakr, M.D., Ph.D.
    Assistant Professor
    Phone: (251) 460-6704
    Fax: (251) 460-6798
    aalmehdi@jaguar1.usouthal.edu


    Research Interests

    • Mechanotransduction by vascular endothelial cells with flow-adaptation and flow-cessation (ischemia). Shear stress sensors on pulmonary endothelial cells. Endothelial cell reactive oxygen/nitrogen species (ROS/RNS) generation during oxygenated-ischemia in the lung with emphasis on the role of endothelial NADPH oxidase. Role of ROS in cell signaling.

    • Mechanisms of metastasis formation in the lung. Intravascular origin of pulmonary metastasis. Tumor cell-endothelial cell interactions in the pulmonary vasculature.

    Research Techniques

    • Intact-organ fluorescence microscopy of subpleural endothelial cells in situ in isolated, perfused rat and mouse lungs. Use of fluorescent probes for plasma- and mitochondrial membrane potential, intracellular ROS/RNS, calcium, free iron, pH, and K+-channels. Cultured bovine pulmonary artery endothelial cells with or without flow-adaptation. Immunohisto- and cytochemistry, spectrofluoro- and photometry

    EDUCATION

    1987-1990

    Ph.D. (Medicine)

    Crimea Medical Institute, Simferopol, Crimea, Ukraine

    1975-1981

    M.D. (with Honors)

    Crimea Medical Institute, Simferopol, Crimea, Ukraine
      

    PROFESSIONAL EXPERIENCE

    2002-present

    Assistant Professor of Pharmacology,

    University of South Alabama, Mobile, AL

    2000-2001

    Research Assistant Professor of Physiology

    University of Pennsylvania School of Medicine, Philadelphia, PA

    1996-2000

    Research Associate, Institute for Environmental

    University of Pennsylvania, Philadelphia, PA

    1991- 1996

    Postdoctoral Fellow, University of Pennsylvania

    School of Medicine, Philadelphia, PA

    1987-1990

    Predoctoral Fellow, Crimea Medical Institute,
    Simferopol, Crimea, Ukraine

    1984-1986

    Residency in General Surgery, Crimea Medical 

    Institute, Simferopol, Crimea, Ukraine

    1982-1984

    Medical Officer, Ministry of Health, Government of

    Bangladesh

    1981-1982

    Intern, Rajshahi Medical College Hospital, Rajshahi,

    Bangladesh

       

    AWARDS, HONORS

    1997 - 2000

    Parker B. Francis Fellowship in Pulmonary Research

    ($108,000).

    1993 - 1995

    National Research Service Award in Respiratory

    Physiology, NIH

    1984 - 1990

    National Scholarship, Ministry of Education, Bangladesh  

    1974 - 1981

    National Scholarship, Ministry of Education, Bangladesh  

    1970 - 1974

    Merit Scholarship, Secondary and Higher Secondary

    Education Board, Rajshahi, Bangladesh

          
    MEMBERSHIP

    American Physiological Society

    American Association for Cancer Research
     

    COURSES TAUGHT DURING THE LAST TWO YEARS   

    Pharmacology Block VI.
     

    PRINCIPAL INVESTIGATOR OF RESEARCH GRANTS

    NIH SBIR

    PI of Project: Contrast agents for Far-Red and Near-IR Imaging

    10/01-9/03 $67,000
     

    NIH CAM SCOR in HBO2 Therapy P50 AT00428-01

    Leader of Project 3: Effect of normo- and hyperbaric hyperoxia on the fate of tumor cells and leukocytes in the pulmonary circulation.

    9/00-8/05 $600,000
     

    Susan G. Komen Breast Cancer Foundation

    99-003201: Fate of Breast Tumor Cells in Lung Vessels.

    12/99-12/01 $233,990.
     

    American Lung Association

    RG 5-21337: Role of membrane potential in lung ischemia/reperfusion injury.

    7/95-6/97 $50,000.
     

    Pennsylvania Lung Association

    RG 5-21676: Role of membrane potential in lung ischemia/reperfusion injury.

    7/94-6/95 $25,000.
     

    EDITORIAL POSITIONS

    Ad hoc Reviewer: Am. J. Resp. Cell Mol. Biol.;  Am. J. Resp. Crit. Care Med.

    J. Appl. Physiol.; AJP Lung, Free Radical Biol. Med., Circ. Res., Cancer Lett.

     

    PUBLICATIONS

    1. Fu W, Z Zhou, SA Santoro, CM Dipersio, A.B. Al-Mehdi, RJ Muschel. The integrin a3b1 mediates attachment of tumor cells to the pulmonary endothelium. Submitted.

    2. Qiu H, FW Orr, D Jensen, H Wang, AR McIntosh, BB Hasinoff, DM Nance, S Pylypas, C Song, RJ Muschel, A.B. Al-Mehdi. Arrest of B16 melanoma cells in the mouse pulmonary microcirculation induces eNOS-dependent nitric oxide release that is cytotoxic to the tumor cells. Am. J. Pathol. Submitted.

    3. Roy A., Jinqing Li, A.B. Al-Mehdi, Anil Mokashi, and Sukhamay Lahiri. Effect of acute hypoxia on glomus cell Em and øm as measured by fluorescence imaging. J. Appl. Physiol. [In Press].

    4. Wong, C, A. Lee, L. Shientag, J. Yu, Y. Dong, G. Kao, A.B. Al-Mehdi, E.J. Bernhard, R.J. Muschel. Apoptosis: An Early Event in Metastatic Inefficiency. Cancer Res. 2001; 61: 333-338.

    5. Wong, CW., C. Song, MM Grimes, W Fu, MW Dewhirst, RJ Muschel, A.B. Al-Mehdi. Intravascular Location of Breast Cancer Cells after Spontaneous Metastasis to the Lung. Am. J. Pathol. September, 2002.

    6. Wei Z., A.B. Al-Mehdi, A.B. Fisher. Signaling pathway for nitric oxide generation with simulated ischemia in flow-adapted endothelial cells. Am. J. Physiol. Heart Circ. Physiol. 2001; 281: H2226-2232.

    7. Song C., A.B. Al-Mehdi, A.B. Fisher. An immediate endothelial cell signaling response to lung ischemia. Am. J. Physiol. Lung Cell. Mol. Physiol. 2001; 281:L993-L1000.

    8. Manevich Y, A.B. Al-Mehdi, V. Muzykantov,  A. B. Fisher. Oxidative burst and NO generation as the initial response to "ischemia" in flow-adapted endothelial cells. Am. J. Physiol. Heart Circ Physiol. 2001; 280: H2126-2135.

    9. Wong, C.W., A. Lee, L. Shientag, J. Yu, Y. Dong, G. Kao, A.B. Al-Mehdi, E.J. Bernhard, R.J. Muschel. Apoptosis: An Early Event in Metastatic Inefficiency. Cancer Res. 2001; 61: 333-338.

    10. Al-Mehdi , AB , C. Song, K. Tozawa, A.B. Fisher. Ca2+- and PI3 kinase-dependent nitric oxide generation in lung endothelial cells in situ with ischemia. J. Biol. Chem. 2000; 275:39807-39810.

    11. Haj-Yehia, A., T. Nassar, B.S. Sachais, A. Kuo, K. Bdeir, A.B. Al-Mehdi, A. Mazar, D.B. Cines, A.A. Higazi. Urokinase derived peptides regulate vascular smooth muscle cell contraction in vitro and in vivo. FASEB J. 2000 14: 1411-1422.

    12. Roy, A., C. Rozanov, A. Mokashi, P. Daudu, A.B. Al-Mehdi, H. Shams and S. Lahiri. Mice lacking in gp91phox subunit of NAD(P)H oxidase showed glomus cell [Ca2+]i and respiratory responses to hypoxia. Brain Res. 2000; 872:188-193.

    13. Al-Mehdi, A. B., G. Zhao, K. Tozawa, A.B. Fisher. Depolarization-associated iron release with abrupt reduction in pulmonary endothelial shear stress in situ. Antiox. Redox. Signal. 2000; 2:345-355.

    14. Al-Mehdi, A.B., K. Tozawa, A.B. Fisher, L. Shientag, A. Lee, R.J. Muschel. Intravascular origin of metastasis from the proliferation of endothelium-attached tumor cells: a new model for metastasis. Nature Medicine, 2000; 6:100-102.

    15. Wei, Z., K. Costa, A.B. Al-Mehdi, C. Dodia, V. Muzykantov, A. B. Fisher. Simulated ischemia in flow-adapted endothelial cells leads to generation of reactive oxygen species and cell signaling. Circ Res 1999 85: 682-689.

    16. Tozawa K., A. B. Al-Mehdi, V. Muzykantov, A. B. Fisher.In situ imaging of intracellular calcium with ischemia in lung subpleural microvascular endothelial cells. Antiox. Redox Signal. 1999; 1: 145-154.

    17. Al-Mehdi, A.B., A.B. Fisher. Invited Editorial on “Tumor Necrosis factor-a in ischemia and reperfusion injury in rat lungs”. J. Appl. Physiol. 1998; 85: 2003-2004.

    18. Al-Mehdi, A. B., G. Zhao, C. Dodia, K. Tozawa, K. Costa, V. Muzykantov, C. Ross, F. Blecha, M. Dinauer, and A. B. Fisher. Endothelial NADPH oxidase as the source of oxidants in lungs exposed to ischemia or high K+. Circ. Res. 1998; 83: 730-737.

    19. Al-Mehdi, A.B., G. Zhao, and A.B. Fisher. ATP-independent membrane depolarization with ischemia in the oxygen-ventilated isolated rat lung. Am. J. Respir. Cell Mol. Biol. 1997; 18:653-661.

    20. Zhao G., A.B. Al-Mehdi, A.B. Fisher. Anoxia/reoxygenation versus ischemia in isolated rat lungs. Am. J. Physiol. Lung Cell. Mol. Physiol. 1997; 273:L1112-L1117.

    21. Atochina, E.N., V.M. Muzykantov, A.B. Al-Mehdi, S.M. Danilov,  A.B. Fisher.  Normoxic lung ischemia/reperfusion accelerates shedding of angiotensin converting enzyme from the pulmonary endothelium. Am. J. Respir. Crit. Care Med. 1997; 156:1114-1119.

    22. Al-Mehdi, A.B., H. Shuman, A.B. Fisher. Intracellular generation of reactive oxygen species during non-hypoxic lung ischemia. Am. J. Physiol. Lung Cell. Mol. Physiol. 1997; 272:L294-L300.

    23. Fisher, A.B.,  A.B. Al-Mehdi. Manifestations and mechanisms of ischemia/reperfusion injury to the lung. In: Oxygen, Gene Expression, and Cellular Function. Clerch, L.B. and Massaro, D.J, eds. Marcel Dekker, Inc., New York , NY , Chapter 14, pp. 309-338, 1997.

    24. Al-Mehdi, A.B., H. Shuman, A.B. Fisher. Oxidant generation with K+-induced depolarization in the isolated perfused lung. Free Rad. Biol. Med. 23:47-56, 1997.

    25. Al-Mehdi, A.B., H. Ischiropoulos, A.B. Fisher. Endothelial cell oxidant generation during K+-induced membrane depolarization. J. Cell. Physiol. 1996; 166:274-280.

    26. Fisher, A.B., A.B. Al-Mehdi. Lipid and protein oxidation in ischemia/reperfusion of the lung. In: Nitric Oxide and Radicals in the Pulmonary Vasculature (ed: Weir, E.K., Archer, S.L., And Reeves, J.T.) Futura Publishing Co, Inc., Armonk , N.Y. 1996; pp 45-61.

    27. Ischiropoulos, H., J. Nelson, D. Duran,  A.B. Al-Mehdi. Reactions of nitric oxide and peroxynitrite with organic molecules and ferrihorseradish peroxidase: interference with the determination of hydrogen peroxide. Free Rad. Biol. Med. 1996; 20:373-381.

    28. Ischiropoulos, H., A.B. Al-Mehdi. Peroxynitrite-mediated oxidative protein modifications. FEBS Letters 1995; 364:279-282.

    29. Ischiropoulos, H., A.B. Al-Mehdi, A.B. Fisher. Reactive species in ischemic rat lung injury: Contribution of peroxynitrite. Am. J. Physiol. Lung Cell. Mol. Physiol. 1995; 13:L 158-L 164.

    30. Fisher, A.B., C. Dodia, A.B. Al-Mehdi, H. Ischiropoulos. Ischemia/reperfusion injury in the rat lung: modulation by K+-channel activity. In: The Oxygen Paradox (ed: Davies, K.J.A., and Ursini, F.) Cleup University Press, Padua , Italy . 1995; pp 708-720.

    31. Al-Mehdi, A.B., H. Shuman, and A.B. Fisher. Fluorescence microtopography of oxidative stress in lung ischemia-reperfusion. Lab. Invest., 1994; 70:579-587.

    32. Fisher, A.B., C. Dodia, I. Ayene,  A.B. Al-Mehdi. Ischemia/reperfusion injury to the rat lung. Ann. N.Y. Acad. Sci. 1994; 723:197-207.

    33. Al-Mehdi, A.B., C. Dodia, M.Jain, A.B. Fisher. A phospholipase A2 inhibitor decreases generation of thiobarbituric reactive substance during lung ischemia-reperfusion. Biochim. Biophys. Acta 1993; 1167:56-62.

    34. Ayene, S.I., A.B. Al-Mehdi, A.B. Fisher. Inhibition of lung tissue oxidation during ischemia/reperfusion by 2-mercaptopropionylglycine. Arch. Biochem. Biophys. 1993; 303:307-312.