EDUCATION

EMPLOYMENT

AWARDS / HONORS

OTHER ACTIVITIES

1992-present Ad Hoc reviewer: NSF, NIAID, NIH; currently on AHA.
 

REVIEWER

Virology, Journal of Virology, BioTechniques, Biochemical Pharmacology, European Journal of Biochemistry, EMBO J., etc.
 

MEMBERSHIPS

Full Member, American Society for Virology.
Member, South-Eastern Society of Parasitologists.
Member, Lion's-USA Eye Research.
 

RESEARCH INTEREST

Interaction between host cells (mammalian cells) and pathogenic organisms
(Bacteria: P. gingivalis; Protozoa: Plasmodium and Toxoplasma; Virus: RSV).
 

PUBLISHED PAPERS

Kumar R, Musiyenko A, Barik S (2003) The heat shock protein 90 of Plasmodium falciparum and antimalarial activity of its inhibitor, geldanamycin. Malar J 2:30. 
Burke E, Barik S (2003) Megaprimer PCR: application in mutagenesis and gene fusion.
Methods Mol Biol 226:525-32.
Bitko V, Oldenburg A, Garmon NE, Barik S (2003) Profilin is required for viral morphogenesis, syncytium formation, and cell-specific stress fiber induction by respiratory syncytial virus. BMC Microbiol 3(1):9
Chen X, Ansai T, Barik S, Takehara T (2003) Kinetic and site-directed mutagenesis studies of Prevotella intermedia Acid phosphatase. Protein Pept Lett 10:53-9
Dobson S, Kumar R, Bracchi-Ricard V, Freeman S, Al-Murrani SW, Johnson C, Damuni Z, Chakrabarti D, Barik S (2003) Characterization of a unique aspartate-rich protein of the SET/TAF-family in the human malaria parasite, Plasmodium falciparum, which inhibits protein phosphatase 2A. Mol Biochem Parasitol 126:239-50.
Barik S (2002) Megaprimer PCR. Methods Mol Biol 192:189-96.
Ansai T, Chen X, Barik S, Takehara T (2002) Conserved proline residues near the N-terminus are important for enzymatic activity of class A bacterial acid phosphatases. Arch Biochem Biophys 408:144-6.
Burke E and Barik S (2003) Megaprimer PCR: application in mutagenesis and gene fusion. In "Methods in Molecular Medicine: PCR Protocols", pp. 529-536, Ed. D. Stirling, Humana Press, Totowa, New Jersey, in press.
Kumar R, Adams B, Oldenburg A, Musiyenko A, Barik S (2002) Characterisation and expression of a PP1 serine/threonine protein phosphatase (PfPP1) from the malaria parasite, Plasmodium falciparum: demonstration of its essential role using RNA interference. Malar J 1:5.
Bitko V, Barik S (2001) Phenotypic silencing of cytoplasmic genes using sequence-specific double-
stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses. BMC Microbiol 1:34 (Editor’s Choice List; Press Release)
Dobson S, Kar B, Kumar R, Adams B, Barik S (2001) A novel tetratricopeptide repeat (TPR)-
containing PP5 serine/threonine protein phosphatase in the malaria parasite, Plasmodium falciparum. BMC Microbiol 1:31
Dobson S, Bracchi V, Chakrabarti D, Barik S (2001) Characterization of a novel serine/threonine 
protein phosphatase (PfPPJ) from the malaria parasite, Plasmodium falciparum. Mol Biochem Parasitol 115:29-39
Bitko V, Barik S (2001) An endoplasmic reticulum-specific stress-activated caspase (caspase-12) 
is implicated in the apoptosis of A549 epithelial cells by respiratory syncytial virus. J Cell 
Biochem 80:441-54
Hossain A, Barik S, Kulkarni P (2001) Lack of significant morphological differences between 
human X and Y spermatozoa and their precursor cells (spermatids) exposed to different prehybridization treatments. J Androl 22:119-23
Bracchi-Ricard V, Barik S, DelVecchio C, Doerig C, Chakrabarti R, Chakrabarti D (2000) PfPK6, 
a novel cyclin-dependent kinase / mitogen-activated protein kinase-related protein kinase 
from Plasmodium falciparum. Biochem J 347:255-263 
Burke E, Mahoney NM, Almo SC, Barik S (2000) Profilin is required for optimal actin-dependent 
transcription of respiratory syncytial virus genome RNA. J Virol 74:669-75
Chen X, Ansai T, Awano S, Iida T, Barik S, Takehara T (1999) Isolation, cloning, and expression 
of an acid phosphatase containing phosphotyrosyl phosphatase activity from Prevotella intermedia. J. Bacteriol 181:7107-14
Connor JH, Kleeman T, Barik S, Honkanen RE, Shenolikar S (1999) Importance of the 12-13 
loop in protein phosphatase-1 catalytic subunit for inhibition by toxins and mammalian protein inhibitors. J Biol Chem 274:22366-72
Dobson S, May T, Berriman M, DelVecchio C, Fairlamb A, Chakrabarti D, Barik S (1999) 
Characterization of protein Ser/Thr phosphatases of the malarial parasite: Plasmodioum 
falciparum: Inhibition of the parasitic calcineurin by cyclophilin-cyclosporin complex. 
Mol. Biochem. Parasitol. 99:167-81
Dupuy LC, Dobson S, Bitko V, Barik S (1999) Casein kinase 2-mediated phosphorylation of 
respiratory syncytial virus phosphoprotein P is essential for the transcription elongation activity of the viral polymerase; phosphorylation by casein kinase 1 occurs mainly at Ser215 and is without effect. J Virol 73:8384-92
Hossain A, Selukar S, Barik S (1999) Differential impact of common laboratory treatments on
hypo-osmotic swelling responses of human spermatozoa. J. Assist. Reprod Genet 16:30-4
Burke E, Dupuy L, Wall C, Barik S (1998) Role of cellular actin in the gene expression 
and morphogenesis of human respiratory syncytial virus. Virology 252:137-48
Barik S (1998) Mutagenesis by megaprimer PCR. In "Genetic engineering with PCR" (eds. RM 
Horton and RC Tait), pp. 25-37, Horizon Scientific Press, Wymondham, UK.
Burke E, Barik S (1998) Specific detection of native multi-subunit proteins by slot-blot assay. 
BioTechniques 25:574-82
Connor JH, Quan HN, Ramaswamy NT, Zhang L, Barik S, Zheng J, Cannon JF, Lee EYC, 
Shenolikar S (1998) Inhibitor-1 interaction domain that mediates the inhibition of protein phosphatase-1. J Biol Chem 273:27716-24
Hossain A, Barik S, Rizk B, Huff C, Thorneycroft IH (1998) Morphometric comparison of 
human X and Y spermatozoa under different treatment and conditions. J Androl, pp. 52-53
Hossain A, Barik S, Rizk B, Thorneycroft I (1998) Preconceptional sex selection: Past, present, 
and future. Arch Androl 40:1-12
Hossain A, Barik S, Rizk B, Thorneycroft I (1998) Time-course of hypo-osmotic swelling of 
human spermatozoa: Evidence of ordered transition between subtypes. Hum Reprod 13: 
1578-83
Bitko V, Barik S (1998) Persistent activation of RelA by respiratory syncytial virus involves 
protein kinase C, underphosphorylated IB, and sequestration of protein phosphatase 2A by 
the viral phosphoprotein. J Virol 72: 5610-8
Das A, Barik S, Ghosh B, Whalen W (1997) Immunoprinting: A technique used to study
dynamic protein–nucleic acid interactions within transcription elongation complex. Methods 
Enzymol 274: 363-74
Hossain A, Barik S, Rizk B, Thorneycroft I (1997) Assessment of the quality of frozen 
Sperm by spectrophotometric analysis and sperm bioassay. Arch Androl 39:119-25
Barik S (1997) Mutagenesis and gene fusion by megaprimer PCR. Methods Mol Biol 67:173-81 
Barik S, Taylor RE, Chakrabarti D (1997) Identification, cloning, and mutational analysis of the 
casein kinase 1 cDNA of the malaria parasite, Plasmodium falciparum. J Biol Chem 272: 26132-8
Bichko V, Barik S, Taylor J (1997) Phosphorylation of the hepatitis delta virus antigens. 
J Virol 71: 512-8
Bitko V, Velazquez A, Yang L, Yang YC, Barik S (1997) Transcriptional activation of multiple 
cytokines by respiratory syncytial virus requires activation of NF-B and is inhibited by sodium salicylate and aspirin. Virology 232:369-78
Hossain A, Barik S, Rizk B, Thorneycroft IH (1996) A microscopic approach of sperm 
preparation for intracytoplasmic sperm injection (ICSI). Middle East Fertil Soc J 1: 106-10. Received "Best Paper of the Year" Award from the Journal.
Barik S, McLean T, Dupuy L (1995) Phosphorylation of Ser232 directly regulates the transcriptional activity of the P protein of human respiratory syncytial virus: Phosphorylation 
of Ser237 may play an accessory role. Virology 213: 405-12
Barik S (1995) Site-directed mutagenesis by PCR: substitution, insertion, deletion, and gene 
fusion. Methods in Neurosciences 26: 309-23
Mazumder B, Adhikary G, Barik S (1994) Bacterial expression of human respiratory syncytial 
virus phosphoprotein P and identification of Ser237 as the site of phosphorylation by cellular 
casein kinase II. Virology 205: 93-103
Mazumder B, Barik S (1994) Requirement of casein kinase II-mediated phosphorylation for the 
transcriptional activity of human respiratory syncytial virus phosphoprotein P: transdominant negative phenotype of phosphorylation-defective P mutants. Virology 205:104-11
Barik S (1993) Cloning, expression and biochemical properties of a novel protein serine-threonine 
phosphatase encoded by bacteriophage . Proc Natl Acad Sci USA 90:10633-7
Barik S (1993) The structure of the 5' terminal cap of the respiratory syncytial virus mRNA. 
J Gen Virol 74: 485-90
Pande VN, Kaushik N, Sanzgiri RP, Patil MS, Modak MJ, and Barik S (1993) Site-directed mutagenesis of DNA polymerase I (Klenow) from Escherichia coli : the significance of 
Arg682 in catalysis. Eur J Biochem 214: 59-65
Barik S (1993) Site-specific mutagenesis by double PCR: Megaprimer method. 
Methods Mol Biol 15: 277-86
Barik S, Banerjee AK (1993) Vesiculoviruses: Chandipura, Piry, and Isfahan: 
In Encyclopedia of Virology, eds. R. Webster and R. Granoff (W. B. Saunders)
Barik S, Banerjee AK (1993) Ungrouped rhabdoviruses of birds, mammals, and fish: 
In Encyclopedia of Virology, eds. R. Webster and R. Granoff (W. B. Saunders)
Barik S (1992) Transcription of human respiratory syncytial virus genome RNA in vitro: Requirement of cellular factor(s). J. Virol. 66, 6813-8
Barik S and Banerjee AK (1992). Sequential phosphorylation of the phosphoprotein of vesicular stomatitis virus by cellular and viral protein kinases is essential for transcription activation. 
J. Virol. 66: 1109-18
Banerjee AK, Barik S (1992) Minireview: Gene expression of vesicular stomatitis virus 
genome RNA. Virology 188: 417-28
Barik S, Banerjee AK (1992) Phosphorylation by cellular casein kinase II is essential for 
transcriptional activity of the vesicular stomatitis virus phosphoprotein P. 
Proc Natl Acad Sci USA 89: 6570-4
Takacs A, Barik S, Das T, Banerjee AK (1992) Phosphorylation of specific serine residues 
within the acidic domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro. J. Virol. 66: 5842-8
Banerjee AK, Barik S, De BP (1991) Gene expression of nonsegmented negative-strand RNA 
viruses. Pharmacol Ther 51: 47-70
Barik S, Banerjee AK (1991) Cloning and Expression of vesicular stomatitis virus 
phosphoprotein gene in Escherichia coli: Analysis of phosphorylation status versus 
transcriptional activity. J Virol 65: 1719-26
Takacs A, Perrine KG, Barik S, Banerjee AK (1991) Alteration of specific amino acid residues 
in the acidic domain I of VSV phosphoprotein (P) converts a GAL4-P(I) hybrid into a 
transcriptional activator. New Biologist 3: 581-91
Barik S, Rud EW, Luk D, Banerjee AK, Yong Kang C (1990) Nucleotide sequence analysis 
of the L gene of vesicular stomatitis virus (New Jersey serotype): Identification of 
conserved domains in L proteins of nonsegmented negative-strand RNA viruses. Virology 
175: 332-7
Barik S, Das A (1990) An analysis of the role of host factors in transcription antitermination 
in vitro by Q protein of coliphage lambda. Mol Gen Genet 222:152-6
Barik S, Ghosh B, Whalen W, Lazinski D, Das A (1987) An antitermination protein 
engages the elongating transcription apparatus at a promoter-proximal recognition site. 
Cell 50: 885-99
Barik S, Bhattacharya P, Das A (1985) Autogenous regulation of transcription termination factor 
Rho. J. Mol. Biol. 182: 405-508
Barik S, Mandal NC (1982). Role of S gene product of bacteriophage lambda host in cell lysis. J. 
Biosciences (India) 4: 361-8
Mandal NC, Barik S (1979) Properties of polylysogens containing derepressed N- prophages. 
Molecular Basis of Host-Virus Interactions (ed. M. Chakraborty), Science Press, N. J., 
pp. 555-68
 

SELECTED RECENT ABSTRACTS

Bitko V, Barik S (2000) Respiratory syncytial virus (RSV) infection causes apoptosis of lung 
epithelial cells via a cytoplasmic pathway dependent on casapse-3 and –8 and uncoupled from NF-kappa B activation. Proc. Am. Soc. Virol. 19th Annu. Mtg., p. 157
Dobson S, Chakrabarti D, Barik S (1999) Identification of an aspartic acid rich protein (ARP) as a 
physiological inhibitor of Plasmodium falciparum PP2A. Molecular Parasitology Meeting X, Woods Hole, MA, p. 78
Bracchi V, Barik S, Robert C, Moe D, Chakrabarti D (1999) Functional analysis of a novel CDK-
related protein kinase from P. falciparum. Molecular Parasitology Meeting X, Woods Hole, MA, p. 80
Dobson S, Chakrabarti D, Barik S (1999) Purification and biochemical characterization of 
Plasmodium falciparum serine-threonine type 1 protein phosphatase. Molecular Parasitology Meeting X, Woods Hole, MA, p. 234A
A. Hossain, S. Barik, B. Rizk, and I. H. Thorneycroft (1999) Pattern of DNA amplification in 
human and bull sperm by amelogenin gene-specific primers. 24th Annual Meeting of American Society of Andrology. J. Androl. (suppl.)
A. Hossain, S. Barik, B. Rizk, C. Huff, and I. H. Thorneycroft (1999) Morphometric comparison of 
human X and Y spermatozoa under different treatment and conditions. 23rd Annual Meeting 
of American Society of Andrology. J. Androl. (suppl.)
V. Bitko and S. Barik (1998) Lack of an autocrine effect of cytokines activated by respiratory 
syncytial virus. Proc. Am. Soc. Virol. 17th Annu. Mtg., p. 176
E. Burke and S. Barik (1998) Cellular actin plays an essential role in respiratory syncytial virus 
transcription. Proc. Am. Soc. Virol. 17th Annu. Mtg., p. 117
A. Hossain, S. Barik, B. Rizk, and I. H. Thorneycroft (1998) Practical implications of inclusion of 
hypo-osmotic response sub-types and shorter response evaluation time in hypo-osmotic swelling test (HOST). Annual Meeting of the American Society for Reproductive Medicine. Fertil. Steril. Suppl. 272-273
Bracchi, V., Barik, S., DelVecchio, C., Chakarabarti, R,. and Chakrabarti, D. (1998) PfPK6, an 
unusual CDK/MAP kinase-related protein kinase from Plasmodium falciparum. Molecular 
Parasitology Meeting VIII, Woods Hole, MA, p. 104
Dobson, S., May, T., Berriman, M, Fairlamb, A., Chakrabarti, D., and Barik, S. (1998) 
Serine/Threonine protein phosphatases of Plasmodium falciparum: Inhibition of parasite 
calcineurin by cyclophilin-CsA complex. Molecular Parasitology Meeting IX, Woods 
Hole, MA, p. 424
B. Rizk, S. Barik, A. Hossain, S. Lynn, and I. H. Thorneycroft (1997) Microscopic sperm 
preparation might be a novel approach for intracytoplasmic sperm injection (ICSI). J. Androl. p. 44
L. Dupuy, and S. Barik (1997) Possible role of the non-structural proteins of the respiratory 
syncytial virus in a post-replicative step. Proc. Am. Soc. Virol. 18th Annu. Mtg., p. 96
Barik, S., and Chakrabarti, D. (1997) Stage-specific expression and substrate sequence analysis of 
casein kinase 1 of the malaria parasite, Plasmodium falciparum. Molecular Parasitology 
Meeting VIII, Woods Hole, MA, p. 309
Barik, S., Taylor, R., and Chakrabarti, D. (1996) Identification, expression, and mutational 
analysis of a minimal casein kinase 1 cDNA from Plasmodium falciparum. Molecular 
Parasitology Meeting VII, Woods Hole, MA, p. 102
Ansai, T., Chakrabarti, D., and Barik, S. (1996) Protein phosphatases of the malaria parasite, 
Plasmodium falciparum. Molecular Parasitology Meeting VII, Woods Hole, MA, p. 412
A. Hossain, S. Barik, B. Rizk, and I. H. Thorneycroft (1996) Assessment of the quality of the frozen
stored serum used in Andrology and IVF laboratories. Annual Meeting of the American Society for Reproductive Medicine
Walpita, P., Barik, S., Banerjee, A, and Krous, H. F. (1993) Cloning and sequence analysis of the 
P genes of two recent isolates of human respiratory syncytial virus (subgroup B). IX th 
International Congress of Virology, Glasgow, Scotland, p. 174
Mazumder, B, and Barik, S. (1993) Cloning and expression of the phosphoprotein gene P of 
human respiratory syncytial virus: Analysis of the structure and phosphorylation status of 
the protein. IX the International Congress of Virology, Glasgow, Scotland, p. 256
Barik, S., and Banerjee, A. K. (1992) Phosphorylation of phosphoproteins (P) of nonsegmented 
negative strand RNA viruses by cellular casein kinase 2. ASBMB Fall Symposia, Keystone, Colorado, p. 89
Das, T., Barik, S., and Banerjee, A. K. (1992) Cloning and expression of vesicular stomatitis virus 
nucleocapsid protein N gene in E. coli: Role in the encapsidation of genome RNA in vitro. ASV Annual Meeting, Ithaca, NY, p. 76
Barik, S. (1992) Cloning, expression, and characterization of a protein phosphatase encoded by 
bacteriophage lambda ORF-221. ASBMB Fall Symposia, Keystone, Colorado, p. 84
Barik, S, and Banerjee, A. K. (1990) Cloning and expression of the polymerase (L) protein and 
phosphoprotein (NS) of vesicular stomatitis virus in E. coli: Studies on phosphorylation vs. transcriptional activity. ASV Annual Meeting, Salt Lake City, UT, p. 37
Barik, S, and Banerjee, A. K. (1991) Cellular and L protein kinases regulate transcriptional activity 
of the phosphoprotein (P) of vesicular stomatitis virus. ASV Annual Meeting, Ft. Collins, CO, p. 31
 

PHD STUDENTS

Lesley C Dupuy (1998); Emily Burke (1999); Sean Dobson (2001)

STUDENT ACHIVEMENTS:

Dr. Andrew Velazquez, (Sophomore student), received Clyde G. Higgins Award for best poster in Medical Student Summer Research Program (1995).

Dr. Lesley Dupuy (Graduate student), received Travel Award to attend American Society for Virology Annual Meeting, Bozeman, MT (1997); Travel Award to Eastern Student Research Forum, Miami, FL (1995 and 1996).

Dr. Lesley Dupuy (Graduate Student), awarded Third Prize for Best Overall Oral Presentation in Eastern Student Research Forum, Miami, FL (1995).

Emily Burke, Graduate Student, received Travel Award to Eastern Student Research Forum, Miami, FL (1998); Travel Award to American Society for Virology Annual Meeting, Vancouver, BC (1998).

Naghma Khan, (Sophomore student), received Clyde G. Higgins Award for best research presentation in Medical Student Summer Research Program (2000). 
 

SABBATICAL VISITOR

Visiting Professor Dr. Toshihiro Ansai, DDS, PhD worked in my laboratory on a 1-yr. sabbatical leave from Kyushu Dental College, Japan, on a Japanese Ministry of Education Fellowship (1995-1996).
 

UNIVERSITY COMMITTEES

Chair, Biopolymer Core Advisory Committee (1998-present).
Chair, Research Advisory Committee (1998-present).
Member, Medical Students Admissions Committee (1999-present).
Member, Curriculum Committee (1995- present).
Graduate Preliminary Examination Committee (1995).
Graduate Qualifying Examination (1998).
Co-organizer, Graduate Students' Literature Reports (1998).
Member, Computers and Medical Information Sub-committee (1997-1998).
Member, Biomedical Library Advisory Committee (1996-1997).
Chair, Biomedical Library Advisory Committee (1997-1998).
Member, Biopolymer Core Advisory Committee (1996-1998).
 

RESEARCH FUNDING

NEI / NIH: "Interactions between Ocular tissue and RSV"; R01 (2002-2005).
NIAID/ NIH: "Reversible phosphorylation in Apicomplexans"; R01 (2000-2005).
Burroughs-Wellcome Foundation Award: "New Initiatives in Malaria Research" (1999-2001).
NIAID/ NIH: "Gene expression of human respiratory syncytial virus" (1994-2000).
American Heart Association (1991-1992).
American Cancer Society (1992-1994).