Judy R. Creighton, Ph.D.

1990-1994	B.S., University of West Florida,
	Pensacola, FL
2002-2006	Ph.D., University of South Alabama
	Mobile, AL

2006-2007	Ruth L. Kirschstein Fellowship
	University of California, San Diego
	San Diego, CA
2008-present	Director, Cell Culture Core
	Center for Lung Biology
	University of South Alabama
	Mobile, AL
2009-present	Assistant Professor
	Department of Pharmacology
	University of South Alabama
	Mobile, AL

1994	Outstanding Scholar, University of West Florida
1994	Member Phi Kappa Phi Honor Society

2000	American Association for the Advancement of Science
2003	American Physiological Society
2007	Society for Experimental Biology and Medicine
2008	American Thoracic Society

NIH/NHLBI, P01 HL66299 (Stevens)
Title: "Lung endothelial cell phenotypes"
Dates: 2001-2011
Role: Co-Investigator (Core 2)
Funded: $6,620,000

SUBMITTED/PENDING GRANT SUPPORT

NIH R01 090320
Title: "AMPK activates calcium entry necessary for endothelial barrier repair"
Dates: 2010-2015
Role: Principal Investigator

AHA Scientist Research Development Grant
Title: "AMP-activated kinase promotes calcium dependent endothelial barrier repair"
Dates: 2010-2014
Role: Principal Investigator

PAST GRANT SUPPORT

American Heart Association, Predoctoral Fellowship (AHA 0415112B)
Title: “Adenylyl cyclase 6 and phosphodiesterase 4D4 compartmentalize membrane
cAMP critical for pulmonary microvascular endothelial barrier function”
Role: Principal Investigator
Dates: 2004-2006
Funded: $36,000

1. Experimental Biology, International Convention. Washington, D.C. 1999. “Regulation of cAMP in lung micro- and macrovascular endothelial cells: mechanisms of enhanced barrier function.”
2. Department of Pharmacology. University of California, San Diego. 2006. “PDE4D4 and microvascular permeability.”
3. Experimental Biology, International Convention. San Diego, CA. 2008. “Adenosine monophosphate kinase (AMPK) expression and activity in the lung.”
4. Department of Pharmacology. College of Medicine, University of California, San Diego. 2008. “AMPK expression in the lung.”
5. Integrated Pulmonary Division/CLB Conference. University of South Alabama Mobile, AL. 2009 “AMPK and Barrier Repair.”

Manuscript Review

American Journal of Physiology

UNIVERSITY SERVICE

2005-2006

Graduate Student Representative, Biomedical ScienceProgram Curriculum Committee

CONTRIBUTIONS TO EDUCATION

IDL Methods Course:	2002-current	Contributing Lecturer
Biomedical Enrichment and Recruitment Program:	2003-2004	Contributing Lecturer
Meet the Discipline Days: CLB/Art in Science	2004-current	Presentation

Community

Women in Science: Expanding your Horizons	2003-2005	Workshop
Career Days: Careers in Science	2004	Workshop

1. Stevens T, Creighton J, and Thompson, WJ. Control of cAMP in lung endothelial cell phenotypes. Implications for control of barrier function. Am. J. Physiol., 277 (1 Pt 1), L119-26, 1999.
2. Moore T, Norwood N, Creighton J, Babal P, Brough G, Shasby D, and Stevens T. Receptor dependent activation of store-operated Ca²⁺ entry increases endothelial cell permeability. Am. J. Physiol., 279: L691-L698, 2000.
3. Norwood N, Moore T, Dean D, Bhattacharjee R, Creighton J, Babal P, and Stevens T. Store operated Ca²⁺ entry and endothelial cell permeability. Am. J. Physiol Lung Cell Mol Physiol., 279: L815-L824, 2000.
4. Cioffi DL, Moore TM, Schaack J, Creighton JR, Cooper DM, and Stevens T. Dominant regulation of interendothelial cell gap formation by calcium inhibited type 6 adenylyl cyclase. J. Cell Biol. 157 (7):1267-78, 2002.
5. Creighton J, Masada N, Cooper DMF, and Stevens T. Coordinate regulation of membrane cAMP by Ca²⁺-inhibited adenylyl cyclase and phosphodiesterase activities. Am. J. Physiol Lung Cell Mol Physiol., 284 (1): L100-7, 2003.
6. King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F, and Stevens T. Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes. Microvasc. Research, 67: 139-151, 2004.
7. Wu S, Cioffi E, Alvarez D, Sayner S, Chen H, Cioffi D, King J, Creighton J, Townsley M, Goodman S, and Stevens T. Essential role of a Ca²⁺ selective, store operated current (ISOC) in endothelial cell permeability. Determinants of the vascular leak site. Circ Res. 96:856-863, 2005.
8. Creighton J, Zhu B, Alexyev M, and Stevens T. Spectrin-anchored phosphodiesterase 4D4 restricts cAMP from disrupting microtubules and inducing endothelial cell gap formation. J Cell Sci. 121 (Pt 1):110-9, 2008.
9. Creighton J, Sayner S, Alexeyev M, and Insel P. AMPK and adherens’ junctions: a role for AMPK in endothelial barrier repair. In preparation: 2009.
10. Buford A, Ayers L, Stevens T, and Creighton J. Combinatorial approach for pulmonary endothelial cell isolation. In preparation: 2009.

ABSTRACTS

1. Creighton J, Thompson WJ, and Stevens T. Regulation of cAMP in lung micro-and macrovascular endothelial cells: Mechanisms of enhanced barrier function. FASEB J., 13: A488, 1999.
2. Norwood N, Moore T, Creighton J, Babal P, Bhattacharjee R, and Stevens T. Myosin light chain kinase regulates activation of store-operated calcium entry in pulmonary artery endothelial cells. FASEB J., 13: A502, 1999.
3. Norwood N, Dean D, Moore T, Creighton J, Babal P, and Stevens T. Inhibition of myosin light chain kinase stimulates Ca²⁺ release in pulmonary artery endothelial cells. FASEB J., 13: A503, 1999.
4. Moore T, Norwood N, Creighton J, Babal P, Brough G, and Stevens T. Calcium influx is sufficient but not required for thrombin-induced endothelial cell shape change. FASEB J., 13: A501, 1999.
5. Creighton J, and Stevens T. cAMP accumulation dictates Ca²⁺ inhibition of adenylyl cyclase 6 necessary to increase gap formation in lung microvascular endothelial cells. FASEB J., 14: A693, 2000.
6. Wu S, Brough G, Creighton J, El-Menshawi M, Goodman S, and Stevens T. Disruption of F-actin binding to non-erythroid spectrin induces focal inter-endothelial cell gaps. FASEB J., 14: A694, 2000.
7. Creighton J, and Stevens T. Ca²⁺ inhibition of adenylyl cyclase 6 is regulated by cAMP in lung microvascular endothelial cells. FASEB J., 15: A161, 2001.
8. King J, Weathington T, Creighton J, McDonald F, Gillespie MN, Olson J, Parker J, and Stevens T. Characterization of phenotypically distinct endothelial cell populations from rat lung. FASEB J., 15: A492, 2001.
9. Cioffi D, Creighton J, Cananles K, Cooper DMF, and Stevens T. Expression of a calcium-stimulated adenylyl cyclase prevents thrombin-induced gap formation in microvascular endothelium. FASEB J., 15: A160, 2001.
10. Creighton J, Zhu B, and Stevens T. Functional co-localization of calcium-inhibited adenylyl cyclase and type 4 phosphodiesterase to caveolin-enriched regions controls membrane-associated camp concentrations in lung microvascular endothelium. FASEB J., 18 (4): A716, 2004.
11. Wu S, Creighton J, King J, and Stevens T. Selective disruption of endothelial cell adhesion by activation of a Ca²⁺ selective store-operated current, ISOC. FASEB J., 2002.
12. Creighton J, Zhu B, and Stevens T. Cyclic AMP phosphodiesterase and non-erythroid spectrin interact at regions of cell-cell contact in lung microvascular endothelium FASEB J., 19 (5):A1274, 2005.
13. Creighton J, Zhu B, Alexeyev M, and Stevens T. Cyclic AMP phosphodiesterase 4D4 activity critically maintains membrane cAMP in lung microvascular endothelium. FASEB J., 20 (4) A 748, 2006.
14. Chen H, Chou C, Creighton J, Sellak H, Wu S. Regulation of Cav3.1 T- type calcium channel expression by cAMP in pulmonary microvascular endothelial cells. FASEB J., 20 (4) A749, 2006.
15. Zhu B, Creighton J, Stevens T. Association of cAMP phosphodiesterase with microtubule binding proteins in pulmonary endothelium: the PKA-mediated phosphorylation of Tau and MAP4. FASEB J. 20 (4) A1164, 2006.
16. Creighton J, Zhu B, Alexeyev M, Stevens T. Cyclic AMP phosphodiesterase 4D4 expression in lung endothelium is a determinant of cell phenotype. FASEB J. 21 (6) A1433, 2007.
17. Creighton J, Stevens T, Insel P. Adenosine monophosphate kinase (AMPK) expression in the normoxic and hypoxic lung. FASEB J. 21 (6) A1436, 2007.
18. Creighton J, and Insel P. Adenosine monophosphate kinase (AMPK) expression and activity in the lung. FASEB J. E70, 2008.
19. Buford A, Ayers L, Stevens T, and Creighton J. An efficient combinatorial approach for pulmonary endothelial cell isolation. FASEB J. 2009 23:1024.3.
20. Creighton J. Sayner S, Alexeyev M, and Insel P. AMPK and cell-cell adhesion: a role for AMPK in lung microvascular barrier repair. FASEB J. 2009 23:964.4.