EDUCATION

ACADEMIC POSITIONS

 
SCIENTIFIC and PROFESSIONAL SOCIETIES

American Society for Biochemistry and Molecular Biology
American Association for the Advancement of Science
Association for Research in Vision and Ophthalmology
International Society on Toxinology
American Association for Cancer Research
American Heart Association
 

HONORS, GRANTS and AWARDS           

PUBLICATIONS

1. Honkanen, R. E., M. W. Rigler and J. S. Patton (1985) Dietary fat assimilation and bile salt absorption in the killifish intestine. Am. J. Physiol. 249 (Gastrointest. Liver Physiol. 12):G399-G407.

2. Rigler, M. W., R. E. Honkanen and J. S. Patton (1986) Visualization by freeze fracture, in vitro and in vivo of the products of fat digestion. J. Lipid Research. 27:836-857.

3. Honkanen, R. E. (1986) Intestinal lipid and bile salt assimilation in killifish. Ph.D. Dissertation, University of Georgia.

4. Yousufzai, S. Y. K., R. E. Honkanen and A. A. Abdel-Latif (1987) Muscarinic cholinergic induced subsensitivity in rabbit iris-ciliary body:  effects on myo-inositol trisphosphate accumulation, arachidonate release, prostaglandin synthesis, and contraction. Invest. Ophthalmol. Vis. Sci. 28:1630-1638.

5. Akhtar, R. A., R. E. Honkanen, P. H. Howe and A. A. Abdel-Latif (1987) M2 muscarinic receptor subtype is associated with inositol trisphosphate accumulation, myosin light chain phosphorylation and contraction in sphincter smooth muscle of rabbit iris. J. Pharmacol. Exp. Ther. 243:624-632.

6. Honkanen, R. E. and J. S. Patton (1987) Bile salt absorption in killifish intestine. Am. J. Physiol. 253 (Gastrointest. Liver Physiol. 16):G730-G736.

7. Honkanen, R. E., J. W. Crim and J. S. Patton (1988) Effects of cholecystokinin peptides on digestive enzymes in killifish in vivo. Comp. Biochem. Physiol. 89(A):655-660.

8. Honkanen, R. E. and A. A. Abdel-Latif (1988) AFDX-116 Discriminates between muscarinic M2 receptors of the heart and the iris smooth muscle. J. Mol. and Cellular Biochem. 82:131-135.

9. Honkanen, R. E. and A. A. Abdel-Latif (1988) Characterization of cholinergic muscarinic receptors in the rabbit iris. Biochem. Pharmacol.  37:2575-2583.

10. Abdel-Latif, A. A, S. Y. K. Yousufzai, R. E. Honkanen, S. Tachado and R. A. Akhtar (1989) Polyphosphoinositide hydrolysis and contraction in the iris sphincter smooth muscle: interaction with the cyclic AMP system. In: Proceedings of the International Symposium on Biosignalling in Cardiac and Vascular Systems. M. Fujiwara, S. Narumiya, S. Miwa (eds.) pp. 315-327, Pergamon Press, Oxford.

11. Honkanen, R. E. and A. A. Abdel-Latif (1989) Muscarinic-agonist and guanine nucleotide stimulation of myo-inositol trisphosphate formation in membranes isolated from bovine iris sphincter smooth muscle: effects of short-term cholinergic desensitization. Membrane Biochem. 8:39-59.

12. Honkanen, R. E., E. F. Howard and A. A. Abdel-Latif (1990) M3-muscarinic receptor subtype predominates in the bovine iris sphincter smooth muscle and ciliary processes. Invest. Ophthalmol. Vis. Sci. 31:590-593.

13. Zwiller, J., R. E. Honkanen and A. L. Boynton (1990) Stimulation of DNA synthesis by an inositol polyphosphate activated protein phosphatase in calcium-deprived rat liver cells. Exp. Cell Res. 187:193-196.

14. Zwiller, J., M. O. Revel, A. L. Boynton, R. E. Honkanen and G. Vincedon (1990) Phosphorylation of an inositol phosphate-stimulated protein phosphatase by protein kinase C. Biochem. Internat. 20:967-977.

15. Mordan, L. J., N. M. Dean, R. E. Honkanen and A. L. Boynton (1990) Okadaic acid: a reversible inhibitor of neoplastic transformation of mouse fibroblasts. Cancer Commun. 2:237-241.

16. Honkanen, R. E., J. Zwiller, R. E. Moore, S. L. Daily, B. S. Khatra, M. Dukelow and A. L. Boynton (1990) Characterization of microcystin-LR: A potent inhibitor of Type 1 and Type 2A protein phosphatases. J. Biol. Chem. 265(32):19401-19404.

17. Honkanen, R. E., J. Zwiller, S. L. Daily, B. S. Khatra, M. Dukelow and A. L. Boynton (1991) Identification, purification and characterization of a novel serine/threonine protein phosphatase from bovine brain. J. Biol. Chem. 266(10):6614-6619.

18. Honkanen, R. E., M. Dukelow, R. E. Moore, J. Zwiller, B. S. Khatra and A. L. Boynton (1991) Cyanobacterial nodularin is a potent inhibitor of Type 1 and Type 2A protein phosphatases. Mol. Pharmacol. 40:577-583.

19. Prinsep, M. P., F. R. Caplan, R. E. Moore, G. M. L. Patterson, R. E. Honkanen and A. L. Boynton (1992) Microcystin-LA from a Blue-green Algae belonging to the Stigonematales. Phytochemistry 31(4): 1247-1248.

20. Honkanen, R. E. (1993) Cantharadin, another natural toxin that inhibits the activity of serine/threonine protein phosphatases types 1 and 2A. FEBS lett. 330(3): 283-286.

21. Sjöholm, Å, Honkanen, R. E., and P. O. Berggren. (1993) Characterization of Serine/threonine protein phosphatases in RINm5F insulinoma cells. Bioscience Reports. 13(6): 349-358.

22. Honkanen, R. E., Codispoti, B. A., Tse, K., and A. L. Boynton (1994)  Characterization of natural toxins with inhibitory activity against serine/threonine protein phosphatases. Toxicon. 32(3): 339-350.

23. Honkanen, R. E. and A. L. Boynton. (1994) Serine/threonine protein phosphatases and their inhibitors. In: Protein Kinase C. (J. F. Kuo, ed), Oxford University Press. pp 305-324.

24. Ämmälä, C. Eliasson, L., Bokvist, K., Berggren, P. O., Honkanen, R. E., Sjöholm, Å., and Rorsman, P. (1994) Activation of protein kinases and inhibition of protein phosphatases play a central role in the regulation of exocytosis in mouse pancreatic B cells. Proc. Natl. Acad. Sci. ( USA ).  91:4343-4347.

25. Critz, S. D. and Honkanen, R. E. (1995) Protein phosphatases and their role in the regulation of membrane currents in sensory neurons of Aplysia. Neuroprotocols: a companion to Meth. in Neruobiol. 6(1):78-83.

26. Honkanen, R. E., Caplan, F. R., Baker, K. K., Baldwin, C. L., Bobzin, S. C.,  Bolis, C. M., Cabrera, G. M., Johnson, L. A., Jung, J. H., Larsen, L. K., Levine, I. A., Moore, R. E., Nelson C. S., Patterson, G. M. L., Tschappat, K. D., Tuang, G. D., Boynton, A. L., Arment, A. R., An, J., Carmichael, W. W., Rodland, K. D., Magun, B. E., and Lewin, R. A., (1995) Protein phosphatase inhibitory activity in extracts of cultured blue-green algae (Cyanophyta). J. Phycol. 31:478-486.

27. Sjöholm, Å, Honkanen, R. E., and P. O. Berggren. (1995) Inhibition of serine/threonine protein phosphatase s by secretagogues in insulin-secreting cells. Endrocrinology. 136(8):3391-3397.

28. Honkanen, R. E., Stapleton, J. D., Bryan, D. E., and Abercrombie, J.  (1996) Development of a protein phosphatase based assay for the detection of phosphatase inhibitors in crude whole cell/animal extracts Toxicon 34(11/12): 1385-1392.

29. Honkanen, R. E., Mowdy, D. E., and Dickey, R.W. (1996) Detection of DSP-toxins, okadaic acid, and dinophysis toxin-1 in shellfish by serine/threonine protein phosphatase assay. Journal of AOAC (Association of Official Analytical Chemists) International 79(6):1336-1343.

30. Huang, X., Cheng, A., and R. E. Honkanen. (1997) Genomic organization of the human PP4 gene encoding a serine/threonine protein phosphatase (PP4) suggest a common ancestry with PP2A. Genomics 44: 336-343.

31. Armstrong, S. C., Kao, R., Shivell, L., C., Downey , J. M., Honkanen, R. E., and Ganote, C. E. (1997) Comparison on in vitro preconditioning response of isolated pig and rabbit cardiomyocytes: effects of a protein phosphatase inhibitor, fostriecin. J. Mol. Cell. Cardiol. 29: 3009-3024.

32. Diwan, A. H., Honkanen, R. E., Schaeffer, R. C. Jr., Strada, S. J., and Thompson, W. T. (1997) Inhibition of serine-threonine protein phosphatases decreases barrier function of rat pulmonary microvascular endothelial cells J. Cell Physiol. 171(3):259-270.

33. Walsh, Aimée H., Cheng, A, and Honkanen, R. E. (1997) Fostriecin, an antitumor antibiotic with inhibitory activity against serine/threonine protein phosphatases types 1 (PP1) and 2A (PP2A), is highly selective for PP2A. FEBS letts. 416: 230-234.

34. O. Larsson, C. J. Barker, A. Sjöholm, H. Carlqvist, R. H. Michell, A. Bertorello, T. Nilsson, R. E. Honkanen, G. W. Mayr, J. Zwiller, P. Berggren. (1997) Inhibition of Phosphatases and Increased Ca²⁺Channel Activity by Inositol Hexakisphosphate Science October 17; 278 (5337):471-474.

35. Xizhong Huang and Richard E. Honkanen (1998) Molecular Cloning, Expression and Characterization of a Novel Human serine/threonine protein phosphatase, PP7, that is homologous to Drosophila retinal degeneration C gene product (rdgC). J. Biol. Chem. 273(3):1462-1468.

36. Zhuang Zuo, Nicholas M. Dean, Richard E. Honkanen (1998) Serine/threonine protein phosphatase type 5 acts upstream of p53 to regulate the induction of p21WAF1/Cip1 and mediate growth arrest. J. Biol. Chem. May 15 273 (20). 12250-12258.

37. Weinbrenner, C., Baines, C. P.,  Lui, G-S, Armstrong, S. C., Ganote, C. E., Walsh, A. H., Honkanen, R. E., Cohen, M. V., and Downey, J. M., (1998) Fostriecin, an inhibitor of protein phosphatase 2A, limits myocardial infarct size even when administered after onset of ischemia. Circulation:  98(9):899-905.

38. Cheng, A., Balcozon, R., Zuo, Z, Koons, J., Walsh, A. H. and Honkanen, R. E. (1998)  Fostriecin-mediated G2-M-phase growth arrest correlates with abnormal centrosome replication, the formation of aberrant mitotic spindles, and the inhibition of serine/threonine protein phosphatase activity. Cancer Research: 58(16):3611-9.

39. Wang L., Bhattacharjee A., Zuo Z., Hu F., Honkanen R. E., Berggren P. O., Li M. (1999) A low voltage-activated Ca²⁺ current mediates cytokine-induced pancreatic beta-cell death. Endocrinology 140(3):1200-1204.

40. Zhuang Zuo, Gudrun Urban, Jonathan G. Scammell, Nicholas M. Dean, Tammy K. McLean, Illeana Argon and Richard E. Honkanen (1999) Ser/thr protein phosphatase type 5 (PP5) is a negative regulator of glucocorticoid receptor-mediated growth arrest. Biochemistry, 38 (28):  8849-8857.

41. John H. Connor, Theresa Kleeman, Sailen Barik, Richard E. Honkanen, and Shirish Shenolikar (1999)  Importance of The ß12-ß13 Loop in Protein Phosphatase-1 Catalytic Subunit for Inhibition by Toxins and Mammalian Protein Inhibitors. J. Biol. Chem. 274(32):22366-22372.

42. Xizhong Huang, Mark R. Swingle Richard E. Honkanen. (2000) Photoreceptors Serine/Threonine Protein Phosphatase type 7 (PP7):  cloning, expression and functional analysis. Methods in Enzymology (315): 579-593.

43. Sjoholm A, Honkanen RE (2000). Polyamines regulate serine/threonine protein phosphatases in insulin-secreting cells. Pancreas 20(1):32-37.

44. Aiyang Cheng, Nicolas Dean, and Richard E. Honkanen (2000) Serine/threonine Protein Phosphatase type 1g1 (PP1g1) is Required for the Completion of Cytokinesis in Human A549 Lung Carcinoma Cells. J. Biol. Chem. 275(3):1846-1854.

45. Richard E. Honkanen (2001) Serine/threonine Protein Phosphatases. In: Antisense Drug Technology: Principles, Strategies and Applications, ( Stanley T. Crooke  Editor) Marcel Dekker, Inc. pp. 587-614.

46. Åke Sjöholm, Per-Olof Berggren, and Richard E. Honkanen (2001) Effects of second messengers on serine/threonine protein phosphatases in insulin-secreting cells. Biochem Biophys Res Commun. 283(2):364-8.

47. Gudrun Urban, Teresa Golden, Ileana V. Aragon, Jonathan G. Scammell, Nicholas M. Dean, and Richard E. Honkanen, (2001) Identification of an Estrogen-inducible Phosphatase (PP5) that converts MCF-7 Human Breast Carcinoma Cells into an Estrogen-Independent Phenotype when Expressed Constitutively J. Biol. Chem. 276 929): 27638-27646.

48. Dean, D. A., Urban, G., Aragon , I. V., Swingle, M., Miller, B., Rusconi, S., Bueno, M., Dean, N. M., Honkanen, R.E. (2001) Serine / threonine protein phosphatase 5 (PP5) participates in the regulation of glucocorticoid receptor nucleocytoplasmic shuttling. BMC Cell Biology 2:6 ( 17 May 2001 ).

49. Golden, T., Dean, N. M., and Honkanen, R. E. Use of antisense oligonucleotides: advantages, controls and cardiovascular Tssue.  Microcirculation 2002 9,51-64.

UNITED STATES AND FORIGEN PATENTS

Honkanen, R. E. and Downey , J. W. Method for Treating Cardiovascular Disease Using Protein Phosphatase inhibitors. Serial number 08/941,964; Filing date 10/4/96 , Patent number 5,914242; Issue Date 6/22/99
 

Honkanen, R. E. and Dean, N. Antisense Oligonucleotide Modulation of Human Serine/ Threonine Protein Phosphatase Gene Expression. Serial number 08/975,211; Filing date 11/20/97 , Patent number 5,948,902; Issue Date 9/7/99 Sponsored Research agreement with ISIS Pharmaceuticals. License agreement in progress (confidential).
 

PCT/US98/24824; Filing date 11/19/98 Pending
09/371,252; filing date 8/10/99 Divisional of 08/975,211; pending
 

Honkanen, R. E. Decreasing Cell Proliferation by Decreasing Levels of PP5
 

Serial number 08/975,127; Filing date 11/20/97 , Patent number; pending
 

PCT/US98/24956; Filing date 11/20/98 Pending; Licensed (confidential)
 

Honkanen, R. E. Glucocorticoid Receptor Agonist and Decreased PP5 expression;
 

09/282,736, Filing Date 3/31/99 . Patent No.: 6,235,891 B1; Date of Issue May 22, 2001
 

Honkanen, R.E. Decreasing cell proliferation by decreasing level of PP5;  Australian Patent No. 728674 issued on May 3, 2001 ; expires on November 20, 2018 .
 

ABSTRACTS AND PRESENTED PAPERS

Honkanen, R. E., M. W. Rigler and J. S. Patton (1984) Intestinal absorption and localization of lipids and bile salts in the killifish Fundulus heteroclitus. Gastroenterology 86:1351.
 

Rigler, M. W., R. E. Honkanen and J. S. Patton (1984) Visualization, in vitro, and in vivo, of the products of intestinal fat digestion.  Gastroenterology 86:1355.
 

Honkanen, R. E. and J. S. Patton (1985) A holistic view of bile salt absorption: studies with the killifish using entire small intestines.  Gastroenterology 88:1714.
 

Howe, P. A., R. A. Akhtar, R. E. Honkanen and A.A. Abdel-Latif (1986)  Muscarinic-induced myo-inositol trisphosphate accumulation, myosin light chain phosphorylation and contraction in the rabbit iris sphincter smooth muscle. Twenty First Annual Southeastern Regional Lipid Conference. Cashiers, NC.
 

Abdel-Latif, A. A., S. Y. K. Yousufzai and R. E. Honkanen (1987)  Adrenergic and cholinergic induced subsensitivity and inositol trisphosphate accumulation, arachidonate release and prostaglandin synthesis in rabbit iris-ciliary body. Assoc. Res. Vis. Ophthalmol.  Sarasota , FL.
 

Honkanen, R. E. and A. A. Abdel-Latif (1987) Subclassification of M2  muscarinic acetylcholine receptors in cardiac and iris smooth muscle.  Regulation of Transport Mechanisms: First Conference of Membrane Biophysics Society, Beaufort , NC .
 

Honkanen, R. E., C. L. Lamb, L. E. Gunter and A. A. Abdel-Latif (1988) Characterization of muscarinic acetylcholine receptors in the bovine iris: effects of carbachol desensitization. Assoc. Res. Vis. Ophthalmol. Sarasota , FL.
 

Abdel-Latif, A. A, R. A. Akhtar, R. E. Honkanen, S. Tachado and S. Y. K. Yousufzai. (1988) Polyphosphoinositides, generation of second messengers and contraction in smooth muscle. Symposium; Vascular Signaling, Kyoto , Japan .
 

Honkanen, R. E., E. F. Howard and A. A. Abdel-Latif (1989) Characterization of muscarinic receptors in the bovine iris sphincter using oligo-nucleotides specific for the M1-M4 subtypes. Assoc. Res. Vis. Ophthalmol. Sarasota FL.
 

Mordan, L. J., R. E. Honkanen, A. L. Boynton and N. M. Dean. (1990) Down regulation of the PDGF receptor by the non-phorbol ester tumor promoter okadaic acid. J. Cell. Biochem. Suppl. 14B:311.
 

Honkanen, R. E., J. Zwiller, P. J. Sousa. S. S. Nakamato, M. Y. Kanemitsu, S. L. Daily and A.L. Boynton (1990) A rapid method for the purification of a serine/threonine-specific phosphoprotein phosphatase from the bovine brain:  effects of inositol polyphosphates on enzyme activity. UCLA Symposia on Receptor-Mediated Second Messenger Pathways. Keystone CO .
 

Boynton, A. L., R. E. Honkanen and N. M. Dean. (1990) Possible role of a new class of protein phosphatases, Type 3, in epidermal growth factor regulation. American Society for Cell Biology, San Diego , CA . 1990.
 

Dukelow, M., R. E. Honkanen and A. L. Boynton (1990) Characterization of aquatic natural products which inhibit Type 1 and 2A protein phosphatases. NIH-MBRS-MAAC Symposium Nashville TN.
 

Dean, N. M., Honkanen, R. E., A. L. Boynton and L. J. Mordan (1990) Okadaic acid, a non-phorbol ester tumor promoter, down-regulates the PDGF receptor. American Association for Cancer Research, Washington , D.C.
 

Honkanen, R. E., J. Zwiller, S. L. Daily and A. L. Boynton (1990) Purification of a serine/threonine phosphoprotein phosphatase from the bovine brain which has pharmacological properties similar to both Type 1 and Type 2A phosphatases. ASCB/EMBO Conference "The Cell Cycle" Arlie VA.
 

Honkanen, R. E., M. Dukelow, K. Tse and A. L. Boynton (1991) Serine/threonine protein phosphatase activity in the cell cycle: studies with nodularin, microcystin-LR and okadaic acid. Keystone Symposia on Molecular and Cellular Biology. J. Cell Biochem. (Supp.). Steamboat Springs , CO .
 

Sjöholm, A., R. E. Honkanen, and P-O. Berggren. (1992) Regulation of serine/threonine protein phosphatases in RINm5f insulinoma cells, Diabetologia 35(Supp1):A100.
 

Sjoholm, A., R. E. Honkanen, and P-O. Berggren. (1993) Regulation of serine/threonine protein phosphatases by second messengers in RINm5f insulinoma cells. J. Cell. Biochem. 17A (Supp):315
 

Honkanen, R.E., Codispoti, B. A., Tse, K., and A. L. Boynton. (1993)  Characterization of serine/threonine protein phosphatase inhibitors. J. Cell Biochem. 17A(Supp):310
 

Sjöholm, Å., R. E. Honkanen, and P-O. Berggren (1993) Ca²⁺-Elevating secretagogues inhibit serine/threonine protein phosphatases in insulin-secreting cells. Diabetologia 36(Suppl.) 1: A59.
 

Pancholy, A. S. J. Pittler, Oliveria L. and R. E. Honkanen (1993). Partial nucleotide sequence of a human retinal cDNA encoding a putative serine/threonine phosphatase. Al. Med. 63:(5):
 

Abercrombie J. and R. E. Honkanen. (1994). Detection of okadaic acid, the major toxin associated with diarrhetic shellfish poisoning, in extracts of whole oysters. Al. Med. 63(6):26
 

Sjöholm, Å., R. E. Honkanen, and P-O. Berggren (1994) J. Cell Biochem. Suppl. 18A: 137.
 

Honkanen, R. E. Ku, T. S., and P. Howe. (1994) Effect of EGF and TGF- on SER/THR protein phosphatase activity in CCL64 mink lung epithelial cells. J. Cell Biochem. Suppl.18C: 169.
 

Honkanen, R. E., Zhan, G-L., Bryan , D. E. and Stapleton, J. D. Characterization of serine/threonine protein phosphatases in cultured human ciliary smooth muscle cells. Invest. Opthalmol. Vis. Sci. Suppl. 36:S140, 1995.
 

Diwan, A. H., Honkanen, R.E., Thompson, W. J., and Strada, S. J.  Protein phosphatase (PP) activity in rat pulmonary microvascular endothelial cells (RPMVEC). FASAB J. 9(4): A947, 1995.
 

Honkanen, R. E., Caplan, F R., Patterson, G. M. L. and Abercrombie, J.  Development of a protein phosphatase based assay for the detection of phosphatase inhibitors in crude whole cell/animal extracts. Toxicon, 34(3)307-308, 1996.
 

Diwan, A. H., Honkanen, R. E., Schaeffer Jr., R. C., Strada, S. J., and Thompson, W. J. Regulation of microvascular endothelial permeability by protein phosphatases. FASAB J. 10(3): A413, 1996.
 

Honkanen, R. E., Balczon, R., Stapleton, J., Bryan D., and Cheng, A. Cantharidin, an Inhibitor of Certain Okadaic Acid Sensitive Serine/Threonine Protein Phosphatases, Blocks Normal Mitotic Progression and Produces Spindle Apparatus with Multiple Poles in Chinese Hamster Ovary Cells. Cancer Susceptibility Genes and Molecular Carcinogenesis. Supp. A13, 1996.
 

Honkanen, R. E., and Dickey, R.W. Assessment of a Serine/Threonine protein phosphatase based assay for the detection of DSP-toxins in shellfish. FASAB J. 10(6):A1459, 1996.
 

Honkanen, R. E., Cheng A. Koons, J. S. and Balczon, R. Fostriecin, an antitumor drug with potent inhibitory activity against certain protein phosphatases, blocks normal mitotic progression and promotes the dissociation of centrosome replication from DNA synthesis.Mol. Biol. Cell. 7 SS, 3273, 1996.
 

Honkanen, R. E., Protein phosphatases: the “yang” of phosphorylation mediated signaling pathways in cells. Fort Johnson Marine Science Seminar Series. Invited oral presentation 1996.
 

Sjöholm, Å, Cook, G. A., and R. E. Honkanen. Inactivation of beta-cell protein phosphatase and carnitine palmitoltransferase 1 by glycocolytic and Krebes cycle intermediates, ATP, GTP, glutamate, and sulfonylurea. Diabetes European Society, Vienna . 1996.
 

Tran, M. Dean, D. A., Lausch, R.L., Honkanen, R. E. and Oakes, J. E. HSV-1 infection of human corneal epithelial cells does not induce release of intracellular stores of IL-1a ARVO 1997.
 

Honkanen, R. E. (1997) The role of serine/threonine protein phosphatases in cell cycle regulation, Cleveland Clinic Foundation, Cleveland OH : Invited oral presentation.
 

Honkanen, R. E. (1997) Serine/threonine protein phosphatase inhibitors and the cell cycle. Gordon Research Conference, Mycotoxins and Phycotoxins, New England College , Henniker , New Hampshire (June 15-20) Invited oral presentation. 
 

Honkanen, R. E. (1997). Serine/threonine Protein Phosphatases the “Yang” of protein phosphorylation mediated signal transduction mediating cell growth and proliferation. ISIS Pharmaceutical. Invited oral presentation.
 

Weinbrenner, C., Baines, C. P., Honkanen, R. E., Cohen, M. V., and Downey , J. M., (1997) Ischemic Preconditioning in the rabbit heart does not protect by inhibiting PP1 or PP2A. American heart association 70^th session.
 

Honkanen, R.E., (1997) Lipoprotein metabolism, Lipid preceptor ship, sponsored by Park-Davis, Mobile Al.
 

Zuo, Z., Wang, L., Li, M., and Honkanen, R. E. (1997) Fostriecin, a Protein serine/threonine phosphatases inhibitor causes a decrease of calcium current and apoptosis in rat insulin-secreting cells. 17^th International Congress of Biochemistry and Molecular Biology. Seattle, WA.
 

Huang, Z., Cheng, A., and Honkanen, R. E., (1997) Genomic organization of the human PP4 gene encoding a serine/threonine protein phosphatase (PP4) suggest a common ancestry with PP2A. 17^th International Congress of Biochemistry and Molecular Biology. Seattle , WA.
 

Honkanen, R.E. (1997). p21^WAF/CIP1 Regulation by PP5 in A549 lung carcinoma cells; ISIS Pharmaceutical, Carlsbad , CA. Invited oral presentation.
 

Honkanen, R. E. (1998) Application of antisense for the study of cell cycle regulation, Antisense technologies, San Francisco CA; invited oral presentation.
 

Honkanen, R. E., (1998) Serine/threonine protein phosphatase type 5 acts upstream of p53 to regulate the induction of p21WAF1/Cip1 and mediate growth arrest. FASEB Summer Research Conference on Protein Phosphatases, Invited oral presentation.
 

Honkanen, R. E. (1998) The role of serine/threonine protein phosphatase type 5 (PP5) in the regulation of a cellular response to stress, East Tennessee State University . Johnson City , TN Seminar series. Invited oral presentation.
 

Honkanen, R. E. (1999) Regulation of cell cycle progression by Serine/threonine protein phosphatase type 5 (PP5). Pharmacology Seminar Series, Vanderbilt University , Nashville , TN. Invited oral presentation.
 

Honkanen, R. E. Inhibition of Human Ser/Thr Protein Phosphatase expression. (1999) ISIS Pharmaceuticals, Carlsbad CA . Invited oral presentation.
 

Honkanen, R. E. (1999). Serine/threonine protein phosphatase type 5, an emerging star in the regulation of cellular proliferation. Rigel Pharmaceuticals, San Francisco , CA . Invited oral presentation.
 

Honkanen, R. E. (1999). Protein Phosphatase type 5, a key regulator of cellular proliferation. Department of Pharmacology, East Tennessee State University . Johnson City TN , Invited oral presentation
 

Honkanen, R. E. (1999) Ser/thr protein phosphatase type 5(PP5) functions as a negative regulator of glucocorticoid receptor- mediated growth arrest. EMBO Workshop, Euro Phosphatase 99. Leuven , Belgium . Invited oral presentation.
 

Honkanen R. E., Gudrun Urban, Jonathan G. Scammell, Nicholas M. Dean, and (2000) Negative Regulation of Glucocorticoid Receptor-mediated growth arrest by ser/thr protein phosphatase type 5 (PP5). American Association for Cancer Research, San Francisco , CA
 

Gudrun Urban, David A. Dean*, Mark R. Swingle, Nicholas M. Dean and Richard E. Honkanen (2000) Serine Threonine Protein Phosphatase 5 (PP5) Regulates the Nucleocytoplasmic Trafficking of the Glucocorticoid Receptor Complex. FASAB Summer Research Conference; Protein Phosphatases, Copper Mountain CO.
 

Mark R. Swingle and Richard E. Honkanen. (2000) Pharmacological Characterization of Bovine serine/threonine protein phosphatase type 5 (PP5). FASAB Summer Research Conference; Protein Phosphatases, Copper Monition CO.
 

Honkanen, R. E. (2000) Serine/threonine protein phosphatases and the regulation of cell proliferation: PP5 an emerging key regulator of growth. Department of Biological Sciences, Florida State University , Tallahassee FL. Invited oral presentation.
 

Teresa A. Golden Ileana Aragon, Gudrun Urban, Scott R. Cooper, Lynnetta M. Watts, Nicholas M. Dean, and Richard E. Honkanen. (2000) Development of Antisense Oligonucleotides that Specifically Inhibit the Expression of Serine/threonine Protein Phosphatase type 5 (PP5) in vivo. ). FASAB Summer Research Conference: Protein Phosphatases, Copper Mountain CO.
 

Honkanen, R. E. (2000) Serine/threonine protein phosphatases and the regulation of cellular signaling: PP5 an emerging key regulator of both p53 and glucocorticoid mediated growth arrest. Division of Pulmonary and Critical Care Medicine, Northwestern University , Chicago , IL , Invited oral presentation.
 

Richard Honkanen, (2000) Protein Phosphatase Type 5 (PP5), a Regulator of Hormone-Mediated Growth. FASAB Summer Research Conference: Protein Phosphatases, Copper Mountain CO. Invited oral Presentation.
 

Richard Honkanen (2001) An Estrogen Responsive Phosphatase That Converts Human Breast Carcinoma Cells from an Estrogen-Dependent to an Estrogen-Independent Phenotype. Marshal Flight Center (NASA). Huntsville AL . Invited oral Presentation.
 

Richard E. Honkanen (2001) Identification and characterization of an Estrogen Responsive Protein Phosphatase (PP5) that Aids Human Cell Growth and Affords Estrogen-Dependent Cells with the Ability to Proliferate Without Estrogen. Janssen/Rigel protein phosphatase mini-symposium, San Francisco , CA .
 

Richard E. Honkanen, Ileana V. Aragon, Gudrun Urban, Teresa A. Golden, Jonathan G. Scammell, and Nicholas M. Dean (2001) Identification of an Estrogen Responsive Protein Phosphatase That is Critical for Human Breast Carcinoma Cell Growth and Converts MCF-7 Human Breast Carcinoma Cells from an Estrogen-Dependent to an Estrogen-Independent Phenotype When Over Expressed. American Association for Cancer Research; Molecular Biology and New Therapeutic Strategies: Cancer Research in the 21^th Century. Maui , HI . AARC proceedings B:3
 

Richard E. Honkanen, Ileana V. Aragon, Gudrun Urban, Teresa A. Golden, Jonathan G. Scammell, and Nicholas M. Dean (2001) Identification of an Estrogen Responsive Protein Phosphatase (PP5) that Aids Human Cell Growth and Affords Estrogen-Dependent Cells with the Ability to Proliferate Without Estrogen. 11^th International Conference on Second Messengers and Phosphoproteins. Melbourne , Australia , 6: 23
 

Richard E. Honkanen (2001) Protein Phosphatases, more then simple house keeping enzymes. ISIS Pharmaceuticals, Carlsbad CA . Invited oral presentation.
 

Honkanen, R. E, T. Golden, G. Urban, and N. M. Dean (2002) Ser/thr protein phosphatase 5 plays a functional role in the apoptosis / growth suppression decision of human A549 lung carcinoma cells following cellular stress. American Association for Cancer Research: Apoptosis and Cancer, Waikola , HI .
 

Kathy and Richard E. Honkanen (2002) Exploring the Molecular Mechanism Associated with the Anti-tumor activity of Cantharidin. FASAB Summer Research Conference: Protein Phosphatases, Snowmass , CO . p. 18.
 

Teresa Golden, Ileana V. Aragon, Gudrun Urban, Nicholas M. Dean and Richard E. Honkanen, (2002) Both Ser/thr protein phosphatase 5 and p53 play a functional role in glucocorticoid receptor-mediated growth control of human cells. FASAB Summer Research Conference: Protein Phosphatases, Snowmass, CO. p. 16.
 

UNIVERSITY SERVICE

Medial School admissions committee 1996-2000
Institutional Bio-safety Committee
Cancer Center Curriculum committee 2000-
Chairman of Biomedical Library committee, 1998-2001
Elected to the Faculty Senate
Salaries and Fringe benefits, subcommittee of the faculty senate
Research cytometry Laboratory  Advisory committee 1999-
Distinguished Speaker Seminar Committee 1993-1996, 1999-2002
Institutional Animal Care and use committee 1999-2002
Student Research Committee 1999-2002
Chair, Medical Student Summer Research Committee 2001
Cancer Institute: Basic/Translational Science Task Force 2001-
Cancer Biology Adviaroy Committee 2000-
LCME Clinical Science Department Subcommittee 2001-
LCME Objective subcommittee 2001-
Student Evaluation Committee representative of Biochemistry 2000-2002
Student Evaluation Committee representative of Cancer Biology 2001-2003
Patent Committee (Chair) 2001-2003
University Radiation Safety Committee 2000-2003
Medical Student Summer Researhc Committee 2002-
Some other committee with Graduate students- Spec  Tony Gard Chair
Committee on committees (2002-)
 

TEACHING RESPONSIBILITY

BCM 121 Medical Biochemistry; Spring; lipoprotein metabolism, system based case studies for freshman medical students.
BCM 471 Research in Biochemistry and Molecular Biology, Medical Student elective
BCM 525 Introduction to Biochemistry Research; Fall, Winter and Spring; hours/quarter; Graduate students, training
BCM 526 Biochemistry Literature Reports, Graduate students.
PHA 641 Molecular and Cellular Pharmacology, Graduate students
Molecular Neurobiology, Graduate students
BCM 620 Enzymes and Proteins; Graduate students
BCM 622 Molecular Biology, Graduate students
BCM 527 Directed Studies in Biochemistry, Undergraduate students 
BCM 626 Biochemical Research Seminar, Graduate students
Summer Research (Medical Students) 
  

THESIS/DISSERTATION SUPERVISION AND ADVISORY COMMITTEES

Major Professor:
       Aiyang Cheng, Biochemistry Ph. D., 1999
       Xizhong Huang, Biochemistry Ph. D., 1998
       Zhuang Zuo, Biochemistry Ph. D., 1998
       Mark Swingle, MS, 1999
       Mark Swingle Ph. D. student 
       Ni Li. Ph. D. student
       Guofei Zhou Ph. D. student
  

Advisory Committees:
       Hafax Diwan, Pharmacology, Ph.D. 1996
       Leah Gillis, Biochemistry Ph. D., 1997
       Yuan Liu, Biochemistry Ph. D., 1997
       Lesley Dupuy, Biochemistry Ph. D., 1998
       Bei Liu, Biochemistry Ph. D., 1999
       Emily Burke, Biochemistry Ph. D., 1999
       Xiaotian Yan, Microbiology, Ph.D. 1999
       Mau Tran Microbiology, Ph. D. 1999
       Scott Hollensworth, Ph. D. 1999 
       Christopher Baines, Ph. D. 1999
       Sean Dobson, Ph. D. 2001
       Cathy Web, Ph. D. expected 2003
       Inna, Shokolenko, Ph.D. expected, 2002
       Wesley Denny, Ph.D. expected 2002
       Atef Khalaf Syed, Ph. D. expected 2003
       Tina Hubler, Ph. D. expected 2004
       Hope Richard, Ph. D. expected 2004
       Jeff Breit, Ph. D. expected 2005
 

Ad Hoc reviewer for:
       Biochemistry
       J. Biol. Chem.
       Cell Growth and Differentiation
       Natural Toxins
       Toxicon
 

Grants:

          Albert Heritage Foundation for Medical Research