Wu, Songwei, M.D.
Assistant Professor
Phone: (251) 460-6366
Fax: (251) 460-6798
swu@jaguar1.usouthal.edu

RESEARCH INTERESTS

 American Heart Association Postdoctoral Fellowship Award, 1999-2001
 

American Association for the Advancement of Science, since 12/2003
American Thoracic Society, since 1/2003
American Physiological Society, since 7/2001
  

SELECTED PUBLICATIONS

1. Donna L. Cioffi, Songwei Wu, Mikhail Alexeyev, Steven R. Goodman, Michael X. Zhu and Troy Stevens. Protein 4.1 gates the endothelial store-operated I_SOC calcium channel. Submitted to Journal of Cell Biology.

2. Fengmin Lu, Hairu Chen, Chun Zhou and Songwei Wu. Is there a role for T-type Ca²⁺ channel in glioma cell proliferation? Cell Calcium, 2005 (Forthcoming).

3. Songwei Wu , Eugene A. Cioffi, Diego Alvarez, Hairu Chen, Donna L. Cioffi, Judy King, Judy Creighton, Mary Townsley, Steven R. Goodman and Troy Stevens. Essential role of a Ca²⁺ selective, store-operated current (I_SOC) in endothelial cell permeability. Circulation Research, 2005,96(8): 856-863.

4. David M Rodman, Katherine Reese, Julie Harral, Brian Fouty, Songwei Wu, James West, Marloes Hoedt-Miller, Yuji Tada, Kai-Xun Li, Carlyne Cool, Karen Fagan, and Leanne Cribbs. Low-Voltage-Activated (T-type) Calcium Channels Control Proliferation of Human Pulmonary Artery Myocytes. Circulation Research, 2005, 96(8): 864-872.

5. Viktor Pastukh, Songwei Wu, Craig Ricci, Mahmood Mozaffari and Stephen Schaffer. Reversal of hyperglycemic preconditioning by angiotensin II: role of calcium transport. American Journal of Physiology: Heart and Circulatory Physiology, 2005, 288(4): H1965 - H1975.

6. Judy King, Tray Hamil, Judy Creighton, Songwei Wu, Priya Bhat, Freda McDonald, and Troy Stevens. Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes. Microvascular Research, 2004, 67(2): 139-151.

7. Songwei Wu, Johnson Haynes, Jr., Jame T. Taylor, Boniface O. Obiako, James R. Stubbs, Ming Li and Troy Stevens. alpha 1G T-type Ca²⁺ channels mediate vaso-occlusion of sickled erythrocytes in the lung microcirculation. Circulation Research, 2003, 93(4): 346-353.

8. Songwei Wu, Jose Singerman, Ming Li, George H. Brough, Steven R. Goodman and Troy Stevens. Essential control of an endothelial cell I_SOC by the spectrin membrane skeleton. Journal of Cell Biology, 2001, 154(6): 1225-1234.

9. George H. Brough, Songwei Wu, Donna Cioffi, Timothy M. Moore, Ming Li, Nicholas Dean and Troy Stevens. Contribution of endogenously expressed trp1 to a Ca²⁺ selective store operated calcium entry pathway. FASEB Journal, 2001, 15(10): 1727-1738.

10. Songwei Wu, Timothy M. Moore, George H. Brough, Sherry R. Whitt, Michael Chinkers, Ming Li and Troy Stevens. Cyclic nucleotide gated channels mediate membrane depolarization following activation of store operated calcium entry in endothelial cells. Journal of Biological Chemistry, 2000, 275(25): 18887-18896.

11. Songwei Wu, Min Zhang, Pamela A. Vest, Arin Bhattacharjee, Li Liu and Ming Li. A mibefradil metabolite is a potent intracellular blocker of L-type Ca²⁺ currents in pancreatic beta–cells. Journal of Pharmacology and Experimental Therapeutics, 2000, 292(3): 939-943.

12. Hean Zhuang, Arin Bhattacharjee, Fuquan Hu, Min Zhang, Tapasree Goswami, Lin Wang, Songwei Wu, Per-Olof Berggren and Ming Li. Cloning of a T-type Ca²⁺ channel isoform in insulin-secreting cells. Diabetes, 2000, 49(1): 59-64.

ABSTRACTS

1. Hairu Chen, Fengmin Lu, Bing Zhu, Jan A. van Mourik and Songwei Wu. Ca_V3.1 T-type Ca²⁺ channel mediates Weibel-Palade body exocytosis in lung endothelial cells. FASEB Journal, 2005. 19(5): A1273 #694.1.

2. Donna L. Cioffi, Mikhail Alexeyev, Songwei Wu, Michael X. Zhu, Steven R. Goodman, and Troy Stevens. The essential I_SOC channels is gated by interaction of protein 4.1 with TRPC4. FASEB Journal, 2005. 19(5): A1273 #694.2.

3. Viktor M. Pastukh, Songwei Wu and Stephen W. Schaffer. Role of T-type Ca²⁺ channel in sodium mediated preconditioning . FASEB Journal, 2005. 19(5): A1212 #683.3.

4. Songwei Wu, Judy A.C. King, Sarah L. Sayner, Troy Stevens and Ronald D. Balczon. Disruption of the dynein motor promotes endoplasmic reticulum-plasmalemma coupling important for activation of the Ca²⁺-selective store-operated current in pulmonary microvascular endothelium. FASEB Journal, 2004. 18(4): A715 #467.4.

5. Donna L. Cioffi, Mikhail Alexeyev, Songwei Wu, Michael X. Zhu, Steven R. Goodman, and Troy Stevens. Activation of endothelial I_SOC requires interaction between TRPC4 and protein 4.1. FASEB Journal, 2004. 18(4): A715 #467.5.

6. Songwei Wu, Judy A.C. King, Troy Stevens and Ronald D. Balczon. Close coupling of endoplasmic reticulum to the plasma membrane is required for I_SOC activation in lung microvascular endothelial cells. FASEB Journal, 2003. 17(4): A411 #309.3.

7. Judy A.C. King, Songwei Wu, Donna L. Cioffi, Sarah Sayner and Troy Stevens. Inhibition of type 4 phosphodiesterase induces abluminal translocation of endoplasmic reticulum tubules in lung microvascular endothelial cells. FASEB Journal, 2003. 17(4): A413 #309.13.

8. Eugene A. Cioffi, Brooke Wallace, Donna L. Cioffi, Songwei Wu and Troy Stevens. Molecular modeling of Fura-2-Be²⁺ complexes reveals a distorted p aromatic network that modulates its fluorescence detection: implications for use in living endothelia. FASEB Journal, 2003. 17(4): A414 #309.16.

9. Songwei Wu, Judy Creighton, Judy King and Troy Stevens. Selective disruption of endothelial cell adhesion by activation of a Ca²⁺ selective store-operated current, I_SOC. FASEB Journal, 2002. 16(4): A406 #365.16.

10. Natalie Norwood, Donna Cioffi, Songwei Wu, Steven Dudek, Joe Garcia and Troy Stevens. Ca²⁺ store depletion promotes tyrosine phosphorylation of myosin light chain kinase. FASEB Journal, 2002. 16(4): A467 #365.20.

11. Songwei Wu, Johnson Haynes, Jr., Boniface Obiako, Ming Li and Troy Stevens. T-type Ca²⁺ channel activation contributes to vaso-occlusion of sickled erythrocytes in inflamed lung. FASEB Journal, 2002. 16(4): A407 #365.21.

12. Songwei Wu, Eugene Cioffi, Troy Stevens. Distinct store operated calcium entry pathway in pulmonary macro- and microvascular endothelial cells. FASEB Journal, 2001. 15(4): A161 #167.6.

13. Songwei Wu, George Brough, Nicholas Dean and Troy Stevens. Contribution of endogenously expressed trp1 to a Ca²⁺ selective store operated calcium entry pathway in endothelium. FASEB Journal, 2001. 15(4): A161 #167.7.

14. Natalie Norwood, Songwei Wu, Steven Dudek, Joe Garcia and Troy Stevens. Endothelial cell myosin light chain kinase-1 and activation of store operated Ca²⁺ entry. FASEB Journal, 2001. 15(4): A492 #418.5.

15. Eugene Cioffi, Songwei Wu, Brook Wallace and Troy Stevens.    Modulation of Fura-2 luminescence by alkaline earth cations both in vitro and in vivo in pulmonary macro- and microvascular endothelial cells. FASEB Journal, 2001. 15(4): A492 #418.6.

16. James Taylor, Songwei Wu, George Brough, Ming Li, Troy Stevens. Selective endothelial expression of T-type voltage-gated Ca²⁺ channels in lung microvascular cells. FASEB Journal, 2001. 15(4): A492 #418.7.

17. Troy Stevens, Dermott Cooper, Steven Goodman, Ming Li, Nicholas Dean, Songwei Wu, Judy Creighton, George Brough, Natalie Norwood and Donna Cioffi. Calcium regulation of cAMP production, the control of macro- and micro-vascular endothelial cell function. Journal of Physiology, 2001, 531.P (King's College London - Scientific Meeting): pp5S.

18. Songwei Wu, Min Zhang, Pamela Vest, Arin Bhattacharjee, Li Liu and Ming Li. A mibefradil metabolite is a potent intracellular blocker of L-type Ca²⁺ currents in pancreatic beta–cells. FASEB Journal, 2000, 14(4): A110 #108.21.

19. Songwei Wu, Steven Goodman and Troy Stevens. Non-erythroid spectrin physically links calcium store depletion to activation of calcium entry in endothelial cells. FASEB Journal, 2000, 14(4): A410 #306.13.

20. Songwei Wu, George Brough, Judy Creighton, Marwa El-Menshawi, Steven Goodman and Troy Stevens. Disruption of F-actin binding to non-erythroid spectrin induces focal inter-endothelial cell gaps. FASEB Journal, 2000, 14(4): A694 #484.14.

21. George Brough, Timothy Moore, Songwei Wu, David Dean, Nicholas Dean and Troy Stevens. Endogenously expressed trp1 mediates store operated calcium entry in lung cells. FASEB Journal, 2000, 14(4): A694 #484.15.

22. Songwei Wu, Timothy Moore, George Brough, Ming Li and Troy Stevens. Activation of store operated Ca²⁺ entry causes lung endothelial cell membrane depolarization via a Ca²⁺-inhibited, cAMP-activated K⁺ conducting channel. FASEB Journal, 1999, 13(4): A502 #410.13. 

1. Donna L. Cioffi, Songwei Wu and Troy Stevens. On the endothelial cell I_SOC. Cell Calcium, 2003, 33(5-6):323-336.

2. Songwei Wu. Review of Article “Impairment of store-operated Ca²⁺ entry in TRPC^-/- mice interferes with increase in lung microvascular permeability.” American Thoracic Society – Assemblies: Pulmonary Circulation Journal Club, September 2002, URL: http://www.thoracic.org/assemblies/pc/journal/sept02.asp

1. Songwei Wu , Donna L. Cioffi and Troy Stevens. Receptor-operated Ca²⁺ channels (Chapter 5). In Ion Channels In The Pulmonary Vasculature (Lung Biology in Health and Disease). Jason X-J. Yuan (Ed.). 2005, Taylor & Francis Group. Boca Raton, Florida, 99-124.

2. Donna L. Cioffi, Songwei Wu and Troy Stevens. Transient receptor potential cation channels and store operated Ca²⁺ channels (Chapter 5 ). In Ion Channels In The Pulmonary Vasculature (Lung Biology in Health and Disease) . Jason X-J. Yuan (Ed.). 2005, Taylor & Francis Group. Boca Raton, Florida, 125-146.

1. Calcium signals that mediate adhesion of circulating cells. Grover Conference on the Pulmonary Circulation: Genetic and Environmental Determinants of Pulmonary Endothelial Cell Function, Sedalia, CO, September 10, 2004.

2. Disruption of the dynein motor promotes endoplasmic reticulum-plasmalemma coupling important for activation of the Ca²⁺-selective store-operated current in pulmonary microvascular endothelium. In Featured Topic Session: Hot Topic in Lung Endothelial Biology, Experimental Biology 2004, Washington, DC, April 21, 2004.

3. Close coupling of endoplasmic reticulum to the plasma membrane is required for I_SOC activation in lung microvascular endothelial cells. Experimental Biology 2003, San Diego, CA, April 13, 2003.

4. Selective disruption of endothelial cell adhesion by activation of a Ca²⁺ selective store-operated current, I_SOC. Grover Conference on Proinflammatory Signaling Mechanisms in the Pulmonary Circulation, Sedalia, CO, September 7, 2002.

5. Selective disruption of endothelial cell adhesion by activation of a Ca²⁺ selective store-operated current, I_SOC. In Featured Topic Session: Second Messengers in Lung Cell Function, Experimental Biology 2002, New Orleans, LA, April 24, 2002.

6. T-type Ca²⁺ channel activation contributes to vaso-occlusion of sickled erythrocytes in inflamed lung. Experimental Biology 2002, New Orleans, LA, April 22, 2002.

7. Distinct store operated calcium entry pathway in pulmonary macro- and micro-vascular endothelial cells. Experimental Biology 2001, Orlando, FL, April 1, 2001.

8. Contribution of endogenously expressed trp1 to a Ca²⁺ selective store operated calcium entry pathway in endothelium. Experimental Biology 2001, Orlando, FL, April 1, 2001.

9. Selective endothelial expression of T-type voltage-gated Ca²⁺ channels in lung microvascular cells. Experimental Biology 2001, Orlando, FL, April 2, 2001.

10. A mibefradil metabolite is a potent intracellular blocker of L-type Ca²⁺ currents in pancreatic beta–cells. Experimental Biology 2000, San Diego, CA, April 16, 2000.

11. Non-erythroid spectrin physically links calcium store depletion to activation of calcium entry in endothelial cells. Experimental Biology 2000, San Diego, CA, April 17, 2000.

12. Disruption of F-actin binding to non-erythroid spectrin induces focal inter-endothelial cell gaps. Experimental Biology 2000, San Diego, CA, April 18, 2000.

13. Activation of store operated Ca²⁺ entry causes lung endothelial cell membrane depolarization via a Ca²⁺-inhibited, cAMP-activated K⁺ conducting channel. Experimental Biology 1999, Washington, DC, April 19, 1999.

RESEARCH SUPPORT

1. RO1 HL074116-01A1 Songwei Wu, M.D. (Principal Investigator)          04/01/2004-03/31/2009
   NIH/NHLBI              $1,277,500                                           
   “Lung endothelium in vaso-occlusion”
   Role: Principal Investigator

2. RO1 HL-A60024  Troy Stevens, Ph.D. (Principal Investigator)
   07/01/2002-06/30/2007
   NIH/NHLBI               $1,125,000
   “Store operated Ca²⁺ entry: lung endothelial permeability”
   Role: Co-Investigator

1. Fellowship Award 9920153V Troy Stevens, Ph.D. (Principal Investigator)
   07/01/1999-06/30/2001
   American Heart Association Southeastern Consortium       $50,000
   "Control of lung endothelial cell membrane potential by cyclic nucleotide gated like channel: response to inflammation"
   Role: Fellow

2. Scientist Development Grant  Songwei Wu, M.D. (Principal Investigator)
   07/01/2002-06/30/2006 (Returned on April 1, 2004 )
   American Heart Association National Center      $260,000                       
   “Role of T-type calcium channels in vaso-occlusion of sickled erythrocytes in the lung microcirculation”
   Role: Principal Investigator