Our general use media is used for all of the rat and mouse cells we culture.
ENDOTHELIAL CELL MEDIA
Normal cell culture media contains (DMEM, 10% FBS, 1% Pen/Strep).
500 mL DMEM
50 mL FBS
5 mL pen/strep
The media is usually changed every 3-4 days, by aspirating off old media and replacing with
fresh media (15 mL for T-75 flask).
Media is stored at 4 degrees C, and warmed in a 37 degree C water bath prior to use.
Remember to use only sterile supplies and reagents and to work under a cell culture hood to maintain sterile conditions.
Reagent Vendor catalog #
DMEM Invitrogen 11965
FBS Atlanta Biologicals S11550H
Pen/Strep Invitrogen 15140-122