Bone Marrow:
Specimen Requirements: Indicate peripheral blood
WBC and % blasts on request form. Specimen should be obtained
from the second aspirate. Draw 1-2 cc into a syringe "wetted"
with sodium heparin (500 U heparin/cc). Invert syringe to mix
with heparin. Immediately add specimen to a tube containing 5-10
ml sterile culture medium (e.g. RPMI 1640 or MEM) (tubes available
on request). Mix well by inverting. Cap tightly and cover with
Parafilm. Keep specimen cool (do not freeze). Deliver to laboratory
as soon as possible.
Turn Around Time: 4-7 days. CLL: 8-14 days
Leukemic Blood (for CML, CLL, and APL only):
Specimen Requirements: Please indicate WBC and
percent blasts on request form. Peripheral blood for cancer chromosome
analysis should have 10% or more blasts. 5-10 cc peripheral blood
in a green top tube (sodium heparin). Mix well by inverting. Keep
specimen cool (do not freeze).
Turn Around Time: 4-7 days. CLL: 8-14 days
Solid Tumor:
Specimen Requirements: The amount of tissue
required for analysis is dependent on the tumor type: (For additional
information, please call 1-800-624-1865) Excised tumor tissue
should be immediately immersed in medium (RPMI) in a sterile dish
or container. The tissue should be kept cool (do not freeze).
The tissue should reach the laboratory within 4 hrs., otherwise
the viability of the cells may be compromised resulting in culture
failure.
Turn Around Time: 7-14 days
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