Skin Biopsy:
Specimen Requirements: Biopsy site should be
cleaned using a sterile gauze pad saturated with acetone. DO NOT
use antiseptic soaps or iodine. Allow to dry. If local anesthetic
is used, we recommend a lidocaine solution without epinephrine.
Obtain a piece of skin (full thickness) 3-5 mm in diameter. Transfer
specimen to a tube containing tissue culture medium [preferred]
or sterile isotonic saline solution (tubes available upon request).
Fill tube completely. Cap tightly and seal with Parafilm. Refrigerate
specimen until shipment (do not freeze).
Turn Around Time: 2-3 weeks
Products of Conception/Fetal Tissue/Placenta:
Specimen Requirements: Selected tissue specimens
should be obtained from abortus material. Preferred tissues are
sternal cartilage, skin from the upper arm or thigh, fetal membranes,
cord, or placenta near cord attachment. In the case of an IUFD,
send both fetal tissue and a specimen of placenta. Place specimen
(s) in a tube (s) containing tissue culture medium [preferred]
or sterile isotonic saline solution (tubes available upon request).
Fill tube completely. Cap tightly and cover with Parafilm. Refrigerate
until shipment (do not freeze).
Turn Around Time: 2-3 weeks
Tissue Culture Only (Fibroblast): Long-term
culture of fibroblasts from skin biopsy for use in enzyme analysis,
DNA analysis, etc.
Specimen Requirements: Biopsy site should be
cleaned using a sterile gauze pad saturated with acetone. DO NOT
use antiseptic soaps or iodine. Allow to dry. If local anesthetic
is used, we recommend a lidocaine solution without epinephrine.
Obtain a piece of skin (full thickness) 3-5 mm in diameter. Transfer
specimen to a tube containing tissue culture medium [preferred]
or sterile isotonic saline solution (tubes available upon request).
Fill tube completely. Cap tightly and seal with Parafilm. Refrigerate
specimen until shipment (do not freeze).
Turn Around Time: 2-3 weeks
Cryopreservation of Solid Tissues: Freezing
and storage of cells from solid tissues.
Specimen Requirements: See specimen requirements
of various tissues above.
Thawing and Expansion of Frozen Cells: Thawing
of previously frozen cells and culture of cells to expand the
cell line for further studies.
Specimen Requirements: For use with previously
cryopreserved tissues.
Turn Around Time: 1-2 weeks
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