I am interested in the ‘inverse
problem’ for FRET: given FRET data, what can we know about the distances
between fluorophores in the population?
For example, the inverse problem is
underdetermined. The location of n
particles within a twodimensional membrane has dimension 2n, whereas
typical FRET observations are typically one dimensional (FRET efficiency
verses some parameter of interest) or two dimensional (FRET decay rates show
flouorescence verses time and a parameter of interest).

These two
configurations have the same intermolecular differences and thus would not
be distinguished with FRET.
