Dr. Ronald P. Kiene


Professor, University of South Alabama
Senior Marine Scientist III,Dauphin Island Sea Lab
Ph.D. 1986, SUNY Stony Brook

: Biogeochemistry and microbial ecology; organic sulfur cycling

Research Interests

My research focuses on the role of microorganisms in the cycling of organic matter and important elements such as sulfur and nitrogen in aquatic systems. 
A major focus of my research program is the biogeochemical cycling of dimethylsulfide (DMS) and its precursor dimethylsulfoniopropionate (DMSP) in the marine water column. We carry out this research in local waters but also on oceanographic cruises all over the world. This field continues to broaden as we have discovered new and important roles for DMSP and its degradation products in the marine ecosystem. DMSP is produced by many, but not all, marine algae who use it as an osmotic solute and potentially an antioxidant. DMSP is degraded by microorganisms to volatile DMS which is a major source of sulfur to the atmosphere. This input of DMS to the atmosphere significantly affects atmospheric chemistry (especially the pH of precipitation) and also the global climate system because DMS is oxidized to sulfate aerosols (tiny crystals of salt). Sulfate aerosols affect climate by directly reflecting solar radiation back to space and by serving as cloud condensation nuclei, which in turn affect how clouds reflect solar radiation.
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Current Research Grants

National Science Foundation – Polar Programs-Antarctic Biology and Medicine. Impact of solar radiation and nutrients on biogeochemical cycling of DMSP and DMS in the Ross Sea , Antarctica . (with David Kieber, SUNY ESF).

National Science Foundation – Biocomplexity in the Environment. Complex molecular to global interactions and feedbacks in the marine DMS cycle. (with Patricia Matrai, Bigelow Laboratory, and several other PI’s).

National Science Foundation – Biological Oceanography. Production and dynamics of DMSP and related compounds in response to oxidative stress in marine phytoplankton. (with David Kieber, SUNY ESF ). 

National Science Foundation – Microbial genetics. Bacterial Regulation of Organic Sulfur Cycling in the Ocean: A Genomic Approach.. sub-contract from Univ. Georgia , under grant to Mary Ann Moran.

Environmental Protection Agency – Alabama Center for Estuarine Studies. Oxidative stress protection by dimethylsulfoniopropionate(DMSP) in Spartina alterniflora.


Moran, M.A., A. Buchan, J. M. González, J. F. Heidelberg., W. B. Whitman., R. P. Kiene, J. R. Henriksen, G. M. King, R. Belas, C. Fuqua, L. Brinkac, M. Lewis, S. Johri, B. Weaver, G. Pai, J. A. Eisen, E. Rahe, W. M. Sheldon, W. Ye, T. R. Miller, J Carlton, D. A. R.4, I.T. Paulsen, Q. Ren, S. C. Daugherty, R. T. Deboy, R. J. Dodson, A. S. Durkin, R. Madupu, W.C. Nelson, S. A. Sullivan, M. J. Rosovitz, D. H. Haft, J. Selengut, & N. Ward. Genome Sequence of Silicibacter pomeroyi reveals unique adaptations to the marine environment . Nature, In Press. 
Toole, D.A. D.J. Kieber, R.P. Kiene, E.M. White, J. Bisgrove, D.A. del Valle, D. Slezak. 2004. High dimethylsulfide photolysis rates in nitrate-rich Antarctic waters. 2004. Geophys. Res. Lett.. 31: L11307, doi:10.1029/2004GL019863. 
Vila , M., R. Simó, R.P. Kiene, J. Pinhassi, J.M. González, M. A. Moran, and C. Pedrós-Alió. 2004. Dimethylsulfoniopropionate incorporation by bacterioplankton taxa studied by microautoradiography and fluorescence in situ hybridization. Appl. Environ. Microbiol. 70: 4648-4657. 
Malmstrom, R R., R. P. Kiene, M. Cottrell and D. L. Kirchman. 2004. Contribution of SAR11 bacteria to C, N, and S cycling in the North Atlantic Ocean . Appl. Environ. Microbiol. 70: 4129-4135. 
Keller, M.D., P.M. Matrai, R.P. Kiene, and W. K. Bellows. 2004. Responses of coastal phytoplankton populations to nitrogen additions: Dynamics of cell-associated dimethylsulfoniopropionate (DMSP), glycine betaine ( GBT ) and homarine. Can. J. Fish. Aquat. Sci. 61: 685-699. 
Zubkov, M, L. J. Linn, R. Amann and R. P. Kiene. 2004. Temporal patterns of biological dimethylsulfide (DMS) consumption during laboratory-induced phytoplankton bloom cycles. Mar. Ecol. Prog. Ser. 271: 77-86.
Harada, H, M-A. Rouse, W. Sunda and R. P. Kiene. 2004. Latitudinal and vertical distributions of particle-associated DMSP lyase activity in the western North Atlantic Ocean . Can. J. Fish. Aquat. Sci. 61: 700-711. 
Miley, G.A. and R. P. Kiene. 2004. Sulfate reduction and pore water chemistry in a gulf coast Juncus roemerianus (needlerush) marsh. Estuaries 27: 472-481. 
Malmstrom, R R., R. P. Kiene and D. L. Kirchman. 2004. Identification and enumeration of marine bacteria assimilating dimethylsulfoniopropionate (DMSP) in the north Atlantic and Gulf of Mexico . Limnol. Oceanogr. 49: 597-606. 
Stets, E G, M. E. Hines and R. P. Kiene. 2004. Thiol methylation potential in anoxic, low pH wetland sediments and its relationship dimethylsulfide production and organic carbon cycling. FEMS Microbiology Ecology. 47: 1-11. 
Toole, D.A., D. J. Kieber, R. P. Kiene, D. A. Siegel, and N.B. Nelson. 2003. Photolysis and the dimethylsulfide (DMS) summer paradox in the Sargasso Sea . Limnol. Oceanogr. 48: 1088-1100. 
Moran, M. A., J. M. González, and R. P. Kiene. 2003. Linking a bacterial taxon to organic sulfur cycling in the sea: studies of the marine Roseobacter group. Geomicrobiology Journal. 20:375-388. 
Sunda, W., D. J. Kieber, R. P. Kiene and S. Huntsman. 2002. An antioxidant function for DMSP in marine algae. Nature 418: 317-320. 
Hines , M.E. , K. N. Duddleston, and R.P. Kiene. 2001. Carbon flow to acetate and C 1 compounds in high latitude wetlands. Geophysical Res. Lett. 28: 4251-4254. 
Zubkov, M, B. M. Fuchs, S.D. Archer, R. P. Kiene, R. Amann and P. Burkhill. 2001. Linking the composition of bacterioplankton to rapid turnover of dissolved dimethylsulphoniopropionate in an algal bloom in the North Sea . Environ. Microbiol. 3: 304-311. 
Kiene, R.P. and L. Linn. 2000. The fate of dissolved dimethylsulfoniopropionate (DMSP) in seawater: Tracer studies using 35S-DMSP. Geochim. Cosmochim. Acta. 64: 797-2810. 
Kiene, R.P. and L. J. Linn. 2000. Turnover of dissolved DMSP and its relationship with bacterial production in the Gulf of Mexico . Limnol. Oceanogr. 45(4): 849-861.
Kiene, R. P., L. J. Linn, and J. A. Bruton. 2000. New and important roles for DMSP in marine microbial communities. J. Sea Res. 43: 209-224. 


Current Graduate Students Post Doctoral Associates  Technicians
J. Daniel Husband Doris Slezak Jennifer Meeks
Daniela del Valle