USA Flow Cytometry Core Laboratory
The USA Flow Cytometry Core Laboratory (FCCL), located in the College of Medicine, serves as a college-wide resource available to researchers in the College of Medicine on a subsidized fee-for-service basis. Specifically, the FCCL provides flow cytometry services for both the clinical and basic research faculty.
The FCCL houses a Becton Dickison Canto II Analyzer and a Becton Dickinson FACS Aria
II Analyzer and Cell Sorter.
The Canto II allows the investigator to analyze samples at a reduced cost. Upon successful completion of a basic Operator Course, the Canto II Workstation can be used independently of Flow Core personnel. This allows for after hours experiments to be analyzed. Analysis of samples can also be done with assistance from Flow Core staff during normal business hours. Please call 48 hours in advance for scheduling.
The Aria II Sort instrument allows for more complex analysis. Sorting options include, but are not limited to 1, 2, 3, and 4 way sorts. Call for more information.
Experienced laboratory personnel are available for advice at both the planning and data interpretation stages.
Flow cytometry makes possible the rapid examination of large numbers of cells for their physical properties and/or phenotypic profiles. Cells in suspension are examined with the the flow cytometer, also called a fluorescence activated cell sorter or FACS, are first labeled with a fluorescent antibody or other fluorescent probe. Cells may also be distinguished in the FACS based on their physical properties such as size or granularity.
When investigators bring samples to the FACS, they are usually interested in:
- Quantitation. Cells have usually been labeled with a fluorescent antibody or other probe and the investigator wants to know the percentage represented by the fluorescence-positive cells.
- Sorting. In this case an investigator wants to isolate, i.e., purify, a sub-population of cells.
- Cloning. Cells are placed singly in individual wells of a 96-well microtiter plate.
- Analysis. Biochemical parameters can also be examined with the FACS. For example, modulation of intracellular Ca2+ can be assayed using dyes such as Indo 1 and Fluo 3.
- DNA. Cell cycle distribution, i.e., %G1, S, and G2/M, can be determined with dyes such as propidium iodide.
- Apoptosis. Cells undergoing programmed cell death can be quantitated.
BD Canto II
|Service||USA fee||Non-USA fee|
|Service||USA fee||Non-USA fee|
The FCCL also houses a 6-foot laminar flow tissue culture hood, water bath, cryostat,
seahorse XF24 analyzer and refrigerator.
Seahorse XF24 Analyzer
- International Society of Analytical Cytology (ISAC): Society information, news, journals, and other links.
- Cytometry Mailing List: Also, lists archived messages.
- Univ. of Massachusetts, Amherst: Catalogue of free flow cytometry software including MFI; flow cytometry explanation.
- Molecular Probes: Fluorescent probes and other flow cytometry and fluorescence microscopy reagents.
- Cytometry Servers List at Purdue: Websites, scientific societies, and online journals.
- Beckman-Coulter: Antibodies and other flow cytometry reagents.
- Fluorofinder.com: Fluorofinder streamlines complex multicolor experimental design.