USA Mass Spectrometry Core Facility

USA Mass Spectrometry Core Facility Logo

Overview of lab capabilities

The goal of the USA Mass Spectrometry Core Facility is to support investigators at the University of South Alabama as well as outside institutions and private companies. We provide services such as Protein Profiling, Targeted Proteomics, Untargeted proteomics, Lipidomics, Small molecule identification and quantitation. The facility is located in the USA Mitchell Cancer Institute on the first floor of the research wing. The facility is equipped with two high resolution mass spectrometers including a Thermo LTQ Orbitrap XL, as well as a Thermo Q-Exactive Plus. Also available is a Thermo MSQ Plus mass spectrometer and an Agilent 1200 HPLC (equipped with UV detection).  This facility is supported by the USA College of Medicine, and is a fee for service facility.

Services provided

  • Protein ID from in-gel or solution, using LC/MS/MS, HR-MS/MS
  • Protein ID from complex cell isolates post-translational modifications
  • Global proteomics
  • Metabolite identification and quantitation
  • Lipid analysis
  • Small molecule identification and quantitation

 

▼   Available Equipment

Thermo Q Exactive Plus LC-MS/MSThermo Q Exactive Plus LC-MS/MS

Max Resolution: 120,000
Mass Accuracy: <2ppm

 

Thermo LTQ Orbitrap XL LC-MS/MSThermo LTQ Orbitrap XL LC-MS/MS

Max Resolution: 60,000
Mass Accuracy: <5ppm

 

Thermo MSQ plus LC-MS/MSThermo MSQ plus LC-MS/MS

Available 24/7 to trained users

Agilent 1200 HPLCAgilent 1200 HPLC

UV detection and fractionation

▼   Fee Structure

Sample Preparation

TYPE of ACTIVITY UNITS FEE AMOUNT
Solid phase extraction (with drying) 1.00  $41.00
Liquid-Liquid extraction (with drying) 1.00 $36.00
HPLC purification/fractionation 1.00  $10.00
In-Gel protein digestion 1.00 $123.00
Protein digestion in solution 1.00 $26.00
Cell/vesicle lysis 1.00 $26.00

HPLC Isolation/Separation

TYPE of ACTIVITY UNITS FEE AMOUNT
1 sample + 1 blank 1.00 $19.00

MSQ Chemical Analysis

TYPE of ACTIVITY UNITS FEE AMOUNT
Small molecule m/z, low resolution no fragmentation, Including Reporting 1.00 $34.00

Small Molecule Analysis

TYPE of ACTIVITY UNITS FEE AMOUNT
Small molecule m/z, High Resolution, with or without MS2 Fragmentation, Including reporting 1.00 $105.00

HRMS Analysis

TYPE of ACTIVITY UNITS FEE AMOUNT
Method development Up to 12 hrs $128.00
LTQ Orbitrap XL Instrument, High Resolution 1.00 $99.00
Q-Exactive Plus Instrument, High Resolution 1.00 $122.00

Other

TYPE of ACTIVITY UNITS FEE AMOUNT
Complex project development, requiring design, development and validation 1.00 $1,605.00
Basic bioinformatics and reporting (Dedicated staff time for Data analyses) 1.00 $53.00
Training of users for data analyses   $21.00
Training of users on LC and MSQ   $21.00
▼   Sample Submission

To submit a request for analysis, please fill out a requisition form and email it to Usa.mass@southalabama.edu.

▼   Sourcing/Citing the Lab

Mass spectrometry analysis was performed at the Proteomics and Metabolomics Core Facility at the University of South Alabama.

▼   Instruments Available for Grant Applications

Types of instruments available that can be used by scientists in grant applications

  • Thermo Fisher Orbitrap mass spectrometer
  • High resolution Orbitrap Q Exactive mass spectrometer.
  • Thermo MSQ plus
  • Agilent 1200 LC
  • Software: Xcalibur, MASCOT, Proteome Discoverer
  • LC columns: C3, C18, HILIC
▼   Contact Information

Dr. Marie Migaud Ph.D.
mmigaud@southalabama.edu

Dr. Surendar Tadi
stadi@southalabama.edu

Lindsay Schambeau
lschambeau@southalabama.edu

 

Facility Address:
Mitchell Cancer Institute
Room number 2095
1660 Spring Hill Ave, Mobile, AL 36604

Phone: (251) 445-9860

Email: Usa.mass@southalabama.edu