Table 3. Size of Left Ventricle, Risk Region, and Infarct

 

Heart

LV

Risk Region

Infarct

Risk Region

Infarct

Infarct

 

(mg)

(mg)

(mg)

(mg)

(% of LV)

(% of risk region)

(% of LV)

 

 

 

 

 

 

 

 

Group I

209.46.1

148.45.6

46.94.1

24.02.7=

31.62.7

50.92.6==

16.01.5*#

Group II

153.74.9

110.94.2

45.33.3

24.22.5=

41.02.9

53.23.6==

22.12.4*#

Group III

159.59.4

116.57.9

50.44.0

28.52.8=

43.83.1

56.72.4==

24.62.1*#

Group IV

144.54.1

105.72.6

51.43.7

17.71.5*#

49.34.0

14.21.9*#

17.41.5*#

Group V

195.16.3

130.64.3

46.34.0

23.83.3=

35.83.0

49.43.4=

18.02.3=

Group VI

192.78.7

132.94.7

49.95.3

11.11.9=

38.44.2

22.22.6=

18.71.6=

Group VII

141.57.2

101.45.8

40.05.7

20.23.3=

39.34.7

49.94.0=

19.93.1=

#

Group VIII

159.65.6

115.74.1

48.34.2

11.51.4#

41.83.4

25.93.3#=

10.01.0##

 

Mice in group I (control group, 4-h reperfusion; n=9) underwent a 30-min coronary occlusion followed by 4 h of reperfusion. Mice in group II (control group, 24-h reperfusion; n=14) underwent a 30-min coronary occlusion followed by 24 h of reperfusion. In group III (early PC sham group, n=11), the chest was opened for 1 h before a 30-min coronary occlusion followed by 24 h of reperfusion (the 1 h of open-chest state corresponded to the time interval necessary to perform the six occlusion/reperfusion cycles in group IV). Mice in group IV (early PC group, n=12) were preconditioned with a sequence of six 4-min coronary occlusion /4-min reperfusion cycles; 10 min later, they underwent a 30-min coronary occlusion followed by 24 h of reperfusion. Mice in groups V and VII (late PC sham group, 4-h and 24-h reperfusion; n=13 and 6, respectively) underwent a thoracotomy and 1 h of open-chest state (without coronary occlusion) on day 1 (the 1 h of open-chest state corresponded to the time interval necessary to perform the six occlusion/reperfusion cycles in groups VI and VIII); 24 h later (on day 2), they underwent a 30-min occlusion followed by 4 h (group V) or 24 h (group VII) of reperfusion. Mice in group VI (late PC group, 4-h reperfusion; n=14) were preconditioned with a sequence of six 4-min occlusion/4-min reperfusion cycles on day 1; on day 2, they were subjected to a 30-min occlusion followed by 4 h of reperfusion. Mice in group VIII (late PC group, 24-h reperfusion; n=13) were preconditioned with a sequence of six 4-min occlusion/4-min reperfusion cycles on day 1; on day 2, they underwent a 30-min coronary occlusion followed by 24 h of reperfusion. LV, left ventricle; Heart, total heart weight (ventricles and atria). Data are means SEM.

 

*P<0.05 vs. group III; #P<0.05 vs. group II; =P<0.05 vs. group V; P<0.05 vs. group I; P<0.05 vs. group VII.

 

Reproduced from Ref. 2 with permission.