I am interested in the ‘inverse
problem’ for FRET: given FRET data, what can we know about the distances
between fluorophores in the population?
For example, the inverse problem is
under-determined. The location of n
particles within a two-dimensional membrane has dimension 2n, whereas
typical FRET observations are typically one dimensional (FRET efficiency
verses some parameter of interest) or two dimensional (FRET decay rates show
flouorescence verses time and a parameter of interest).
configurations have the same inter-molecular differences and thus would not
be distinguished with FRET.