Assistant Professor
M.D. (with honors) Crimea Medical Institute
Ph.D. (Medicine) Crimea Medical Institute
Postdoctoral, University of Pennsylvania School
of Medicine |
Mechanotransduction by vascular
endothelial cells with flow-adaptation and flow-cessation
(ischemia). Shear stress sensors on pulmonary
endothelial cells. Endothelial cell reactive oxygen/nitrogen
species (ROS/RNS) generation during oxygenated-ischemia
in the lung with emphasis on the role of endothelial
NADPH oxidase. Role of ROS in cell signaling.
Mechanisms of metastasis formation in the lung.
Intravascular origin of pulmonary metastasis.
Tumor cell-endothelial cell interactions in the
pulmonary vasculature.
Research Techniques
Intact-organ fluorescence microscopy of subpleural
endothelial cells in situ in isolated, perfused
rat and mouse lungs. Use of fluorescent probes
for plasma- and mitochondrial membrane potential,
intracellular ROS/RNS, calcium, free iron, pH,
and K+-channels. Cultured bovine pulmonary artery
endothelial cells with or without flow-adaptation.
Immunohisto- and cytochemistry, spectrofluoro-
and photometry. |
1. Al-Mehdi, A. B., G. Zhao, K. Tozawa,
A.B. Fisher. Depolarization-associated iron release
with abrupt reduction in pulmonary endothelial shear
stress in situ. Antiox. Redox. Signal. 2000 (In
Press).
2. Al-Mehdi, A.B., K. Tozawa, A.B. Fisher, L. Shientag,
A. Lee, R.J. Muschel. Intravascular origin of metastasis
from the proliferation of endothelium-attached tumor
cells: a new model for metastasis. Nature Medicine,
2000; 6:100-102
3. Wei, Z., K. Costa, A. B. Al-Mehdi, C. Dodia,
V. Muzykantov, and A. B. Fisher. Simulated Ischemia
in Flow-Adapted Endothelial Cells Leads to Generation
of Reactive Oxygen Species and Cell Signaling. Circ.
Res. 1999 85: 682-689.
4. Tozawa K., A. B. Al-Mehdi, V. Muzykantov, and
A. B. Fisher.In situ imaging of intracellular calcium
with ischemia in lung subpleural microvascular endothelial
cells. Antiox.RedoxSignal. 1999; 1: 145-154.
5. Al-Mehdi, A. B., G. Zhao, C. Dodia, K. Tozawa,
K. Costa, V. Muzykantov, C. Ross, F. Blecha, M.
Dinauer, and A. B. Fisher. Endothelial NADPH oxidase
as the source of oxidants with lung ischemia or
high K+. Circ. Res. 1998 83: 730-737.
Al-Mehdi, A.B., G. Zhao, and A.B. Fisher. ATP-independent
membrane depolarization with ischemia in the oxygen-ventilated
isolated rat lung. Am. J. Respir. Cell Mol. Biol.
1997; 18:653-661..
6. Zhao G., A.B. Al-Mehdi, and A.B. Fisher. Different
pathways for oxidant generation with anoxia/reoxygenation
versus ischemia/reperfusion in rat lungs. Am.
J. Physiol. Lung Cell. Mol. Physiol. 1997; 273:L1112-L1117.
7. Al-Mehdi, A.B., H. Shuman, and A.B. Fisher.
Intracellular generation of reactive oxygen species
during non-hypoxic lung ischemia. Am. J. Physiol.
Lung Cell. Mol. Physiol. 1997; 272:L294-L300.
8. Al-Mehdi, A.B., H. Shuman, and A.B. Fisher.
Oxidant generation with K+-induced depolarization
in the isolated perfused lung. Free Rad. Biol.
Med. 23:47-56, 1997.
9. Al-Mehdi, A.B., H. Ischiropoulos, and A.B.
Fisher. Endothelial cell oxidant generation during
K+-induced membrane depolarization. J. Cell. Physiol.
1996; 166:274-280.
10. Al-Mehdi, A.B., H. Shuman, and A.B. Fisher.
Fluorescence microtopography of oxidative stress
in lung ischemia-reperfusion. Lab. Invest., 1994;
70:579-587. |
