SSoMES Bioanalytical Center
Our Mission
Email Address:
analytical@southalabama.edu
Phone Number:
251-460-6848
Location:
Stokes School of Marine & Environmental Sciences
Education & Outreach Building
600 Clinic Drive
Mobile, AL 36688
Office Hours:
Monday-Friday
9 am -5 pm
Instrumentation & Equipment
Elemental Analysis (C, H, N, S, O, Hg, Metals)
These instruments directly quantify elemental composition through combustion, thermal decomposition, or plasma ionization.
Milestone DMA-80 Direct Mercury AnalyzerProvides direct determination of total mercury in solids, liquids, and gas without the need for pretreatment of the sample
Combines techniques of decomposition, catalytic conversion, amalgamation, and atomic
absorption spectrophotometry to provide fast and accurate analysis
Working Ranges:
Low Range: 0.003 ng - 20ng
High Range: 20 ng - 1500 ng
Sample Processing Time:
5-8 Minutes
Precision:
<1.5%
Applications
Environmental-determination of mercury in soils, sediments, and waste water
Biomonitoring -determination of mercury in seafood and other potential sources
Energy / Industrial Sector-tracking mercury in combustion byproducts and petrochemical waste streams
Costech CHNS-O ECS 4010Measures total carbon and nitrogen from solid materials such as sediment, plant and animal materials, and GF/F filters
Two furnaces; a combustion furnace up to 1100°C and a reduction furnace at 650°C
Thermal Conductivity Detector (TCD) with chromatographic separation of combustion
gases
Sample Processing time:
5 minutes/sample
Sample capacity per quartz insert:
~30 for filter samples
~50 for sediment/biological sample
Agilent 7700 LA-ICP-Mass SpectrometerProvides precise, elemental analysis of solid and liquid samples at trace and ultra-trace concentrations
Couples laser ablation with high-sensitivity ICP-MS detection to provide quantitative
elemental data on metal ion abundance and speciation in biological samples without
extensive preparation
Detection Range:
Parts per trillion (ppt) to parts per million (ppm)
Sample Processing Time:
Seconds to approximately 5 minutes, dependent on method used
Precision:
Accepted: 20% RSD
Average: <2% RSD
Applications
Environmental- elemental mapping of sediments, soils, and minerals in environmental studies
Biological/ Toxicological- quantifying trace metals in tissues and other biological materials
Materials science- surface composition profiling and contamination analysis
Molecular & Organic Compound Analysis (Mass Spectrometry & Chromatography)
These instruments identify and quantify organic molecules, pollutants, metabolites, and complex mixtures.
Thermo Scientific Orbitrap Exploris 240 Mass SpectrometerHigh-resolution, accurate-mass (HRAM) mass spectrometer used for discovery and quantification of compounds in complex mixtures.
Combines a quadrupole mass filter with Orbitrap detection allowing for targeted and
untargeted analysis of samples with high mass accuracy and resolution across a wide
dynamic range
Mass Range:
40 to 6000 m/z
Max Resolution:
Up to 240,000 (at m/z 200)
Mass Accuracy:
<1 ppm (with internal calibration)
Scan Speed:
Up to 22 Hz
Applications:
Proteomics/ Metabolomics - comprehensive profiling of proteins (tryptic digests), peptides, and metabolites
Environmental/ Toxicological Research- detecting trace organic pollutants and emerging contaminants
Pharmaceutical / Biochemical Research - compound identification.
Accurate mass determination for structural confirmation and characterization;
Other compound discovery e.g., glycomics, lipidomics
Shimadzu QP2010 Gas Chromatography- Tandem Mass Spectrometer
High‐performance gas chromatography–mass spectrometer for both qualitative and quantitative analysis of complex mixtures
Integrates a triple quadrupole mass spectrometer with a versatile gas‑chromatograph
front end, enabling stable, high-quality mass spectra
Mass Range:
1.5 to 1000 m/z
Scan Speed:
10,000 u/sec
Applications:
Environmental / Toxicological - comprehensive profiling of compounds in complex mixtures (e.g., air, soil, water, biological material)
- Dibenzofurans,
- dioxins,
- herbicides,
- sulfur,
- pesticides,
- phenols, and
- PCBs
Manufacturing - qualitative / quantitative analysis of compounds in food, fragrance, and plastics
Chemical / Pharmaceutical / Materials - compound discovery and quantification
Dissolved Carbon, Nutrient, and Water Chemistry Analysis
These instruments quantify dissolved nutrients, carbon fractions, and other aqueous analytes.
Shimadzu TOC-L AnalyzerPerforms direct measurements of dissolved inorganic carbon (DIC), dissolved organic carbon (NPOC), total carbon (TC), and total nitrogen (TN) with minimal pretreatment
Working Ranges:
TC: 0 to 30,000_mg/L
DIC: 0 to 3,000_mg/L
TN: 0 to 10,000_mg/L
Sample Processing Time:
~3 minutes per sample
Precision:
Coefficient of variation (CV) ≤ 1.5%
Applications
Environmental monitoring - organic carbon in surface water, wastewater, and effluent streams
Water‑system cleaning validation and pharmaceutical manufacturing
Skalar San++ Series Continuous Flow AnalyzerProvides automated determination of multiple analytes in liquids including nitrate, nitrite, ammonia, phosphate, and silica without the need for pretreatment
Combines segmented flow analysis and colorimetric detection to provide fast and precise
analysis
Working Ranges
Ammonia:
- Ranges: 0.07-35.7 µM N
- Minimum Detection Limit (MDL): 0.009 µM N
- RSD (%): 0.43%
Nitrate + Nitrite
- Ranges: 0.07-71.4 µM N
- Minimum Detection Limit (MDL):0.005 µM N
- RSD (%): 0.35%
Nitrite
- Ranges: 0.07-71.4 µM N
- Minimum Detection Limit (MDL):0.005 µM N
- RSD (%): 0.38%
Phosphate
- Ranges: 0.03-32.3 µM P
- Minimum Detection Limit (MDL): 0.003 µM P
- RSD (%): 0.35%
Sample Processing Time:
5-10 minutes
Applications:
Environmental - nutrients in water and wastewater systems
Industrial process monitoring - effluents and production streams
Trilogy Fluorometer
Precise optical filtering with stable LED excitation provides accurate, reproducible
results
Measure raw fluorescence as well as direct concentrations
Measurement Time: <10 seconds
Applications
Fluorescence, absorbance, and turbidity measurements across a wide range of applications
Snap-in optical cartridges, allowing users to easily switch between different modules
such as chlorophyll, cyanobacteria, etc.
| Available Modules | ||
|---|---|---|
| GUI Module | Description | Application |
| CHL-A | Chlorophyll-a (Acidified) |
Extracted Chlorophyll-a |
| CHL-A NA | Chlorophyll-a NA (Non Acidified) | Extracted Chlorophyll-a |
| CHL/BLUE | Chlorophyll in vivo | Chlorophyll in vivo |
| CDOM/UV | Chromophoric Dissolved Organic Matter | Carbon Cycle & Nutrient Transport |
| PC/ORANGE | Phycocyanin Orange | Extracted Chlorophyll & Phycocyanin |
| AMMONIUM UV | Ammonium Measurement |
NH4 measurement, Nutrient Transport |
| RWT/PE GREEN | Rhodamine WT fluorescent dye |
Water Flow & Dispersion Mapping |
Thermo Scientific Evolution OnePlus SpectrophotometerHigh‐performance UV-Visible spectrophotometer used for quantitative and qualitative analysis of liquid samples
Dual-beam optical system and high-stability detector provide accurate absorbance measurements
across the UV-Visible spectrum
Wavelength Range:
190 to 1100 nm
Spectral Bandwidth:
1.0 nm
Wavelength Accuracy: ±0.5 nm
Applications:
Wide ranging research applications absorbance, transmittance, and concentration measurements
Molecular research - quantitative analysis of nucleic acids, proteins, and other biomolecules
Environmental / Chemical analyses - colorimetric assays
Sample Preparation
These instruments prepare samples for chemical analysis.
Sterimatic AutoclaveSpecifications:
Temperature settings
110-121 °C / 230-250 °F
Settings:
Fast (Instruments) or
Slow (Liquids)
Automatic timer up to 60 minutes.
84 Liters volume
Capacity 3 cu. ft.
Applications:
Sterilizing equipment, tools, glassware, and media for contaminant-sensitive experiments.
Mettler-Toledo XR2 Microbalance
Elga PureLab Flex MilliQ System
Thermo Dionex ASE 350Extraction Cell Sizes:
1 to 100g
Operating Conditions:
Temperature up to 200 °C
Pressure up to 1500 psi
Applications:
Extraction of hydrocarbons, pesticides, PCBs, PAHs, and other organic contaminants from soil, sediment, plant tissue, fish tissue, and other complex matrices
I
Thermo Lindberg/Blue M Muffle Furnace
Provides precise, high-temperature heating for ashing and heat treatment
Temperature ranges up to 1200 °C with excellent temperature uniformity and digital
PID control for reliable performance
Clean work only*
VWR Drying OvenAllows reliable, uniform heating for drying, curing, and sample preparation with temperature control up to 250 °C
Clean work only*