Dr. Tuan Tran's Research
Areas of interest
· Molecular Plant-Microbe Interactions
· Plant Cell Biology
· Bacterial Pathogenesis
In the Plant-Microbe Interactions research group, we use interdisciplinary approaches to dissect mechanisms that allow plants to maintain membrane integrity and defense response signaling.
The plant plasma membrane is embedded with a plethora of receptors which function as “sensors” to detect subtle changes in the environment, including the presence of plant pathogens. These receptors are organized into nanodomains - specific regions on the plasma membrane that are highly enriched in ordered lipids, such as sphingolipids and sterols. The integrity of these nanodomains have gained much attention lately as there are mounting evidence suggest their direct involvement of these nanodomains in association with immune complex signaling and plant interactions with pathogenic and beneficial microbes. Using interdisciplinary approaches, including advanced microscopy, genetics, and biochemical analyses, we will dissect the functions of plasma membrane constituent and related biological processes involved in plant defense signaling.
Another direction of our research group is to investigate the life of bacteria as biofilms inside plant hosts. Biofilms are structured microbial community that settle on biotic/abiotic surfaces and they play critical roles in a large number of many hospital opportunistic infections caused by clinically important pathogens (Pseudomonas aeruginosa, Staphylococcus spp.). Similar to human pathogens, plant pathogenic bacteria can also reach very high density inside host tissue and complex 3-D structures of biofilms has been reported in several bacterial plant pathogens. The functions of phytopathogenic biofilms, however, are poorly understood. Studying how phytopathogenic bacterial biofilms develop, mature, and spread systematically in planta, therefore, are critical for our understanding of bacterial plant diseases.
Auer S., Haelterman N., Weissgerber T., Erlich J., Susilaradeya D., Julkowska M., Gazda M., Abitua A., Niraulu A., Shah A., Clyburne-Sherin A., Guiquel B., Alicea B., LaManna C., Ganguly D., Perkins E.J., Jambor H., Li I., Tsang J., Kamens J., Teytelman L., Paul M., Phuyal S., Schmelling N., Crisp P., Sarabipour S., Roy S., Bachle S., Tran T.M., Tyler Ford, Steeves V., Ilangovan V., Schwessinger B., Jadavji N. (2021) Science Forum: A community-led initiative for training in reproducible research. eLife
Ma Z., Liu X., Nath S., Sun H., Tran T.M., Yang L., Mayor S., Miao Y. (2021) Formin nano-clustering mediated actin assembly during plant flagellin and DSF-signalling. Cell Reports 34:108884.
Tran T.M.*, Ma Z.*, Triebl A., Nath S., Cheng Y., Gong B., Han X., Wang J., Li J., Wenk M.R., Torta F., Mayor S., Yang L., Miao Y. (2020) The bacterial quorum sensing molecule DSF hijacks host sterol biosynthesis to suppress plant innate immunity. Life Science Alliance. 3(10): e202000720 (*co-first author)
Huang D., Sun Y., Ma Z., Ke M., Cui Y., Wang D., Chen Z., Chen C., Ji C., Tran T.M., Yang L., Lam S.M., Han Y., Shui G., Friml J., Miao Y., Jiang L., Chen X. (2019) Salicylic acid mediated plasmodesmatal closure via Remorin-dependent lipid organization. PNAS 116(42) 21274-21284
Khokhani D., Tran T.M., Lowe-Power T.M., Allen C. (2018). Plant assays for quantifying Ralstonia solanacearum virulence. Bio-protocol. doi: 10.21769/BioProtoc.3028
Khokhani D., Lowe-Power T.M., Tran T.M., Allen C. (2017) A single regulatory switch in a plant pathogen mediates strategic trade-offs between growth/virulence and attachment/spread. mBio 8(5):e00895-17
Tancos M.A., Lowe-Power T.M., Peritore-Galve F.C., Tran T.M., Allen C., Smart C.D. (2017) Plant-like bacterial expansins play contrasting roles in two tomato vascular pathogens. Molecular Plant Pathology. doi: 10.1111/mpp.12611
Tran T.M., MacIntyre A., Hawes M., Allen C. (2016) Escaping underground nets: extracellular DNases degrade plant extracellular traps and contribute to virulence of the plant pathogenic bacterium Ralstonia solanacearum. PLOS Pathogens. 12(6): e1005686. Cover image, featured article, top 50 most downloaded PLOS Pathogens articles in 2016
Tran T.M., MacIntyre A., Khokhani D., Hawes, M., Allen C. (2016) Extracellular DNases of Ralstonia solanacearum modulate bacterial biofilm and facilitate bacterial wilt virulence. Environmental Microbiology. doi10.1111/1462-2920.13446See More
Tran T. M., Jacobs J., Huerta A., Milling A., Weibel J. and Allen C. (2016) Sensitive, secure detection of Race 3 biovar 2 and native U.S. strains of Ralstonia solanacearum. Plant Disease. 100 (3): 630-639. Editor’s pick
Hawes M., Allen C., Gillian Turgeon B., Curlango-Rivera G., Tran T.M., Huskey D., Xiong Z. (2016) Root border cells and their role in plant defense. Annual Review of Phytopathology. 54: 143-161.
Weibel J., Tran T.M., Boczanczy A.M., Daughtrey M., Norman D.J., Mejia L., Allen C. (2016) A Ralstonia solanacearum strain from Guatemala infects diverse flower crops, including new hosts Vinca and Sutera, and causes symptoms in geranium (Pelargonium hortorum), African daisy (Osteospermum ecklonis), and mandevilla (Mandevilla spp.) Plant Health Progress 17(2):114-121.