1. Leavesley, Silas J, Arie Nakhmani, Yi Gao, and Thomas C Rich. “Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification.” cAMP Signaling: Methods and Protocols, 2015, 59–70.  See Cell Profiler example pipeline published at:
  2. Rich, Thomas C, Wenkuan Xin, Silas J Leavesley, and Mark S Taylor. “Channel-Based Reporters for cAMP Detection.” cAMP Signaling: Methods and Protocols, 2015, 71–84.


  1. Silas J. Leavesley, Thomas C. Rich. FRET: Signals hidden within the noise. Cytometry, Part A, 85:11 918-920 (2014)
  2. Taylor MS, Francis M. Decoding dynamic Ca2+ signaling in the vascular endothelium. Front Physiol. 5:447 (2014)
  3. Xu N, Francis M, Cioffi DL, Stevens T. Studies on the resolution of subcellular free calcium concentrations: A technological advance. Am J Physiol Cell Physiol. 06(7):C636-8 (2014)
  4. Francis M, Waldrup J, Qian X, Taylor MS. Automated analysis of dynamic Ca2+ signals in image sequences. J Vis Exp. 88 (2014)
  5. Peter F. Favreau, Clarissa Hernandez, Tiffany Heaster, Diego Alvarez, Thomas C. Rich, Prashant Prabhat, Silas J. Leavesley. An excitation-scanning hyperspectral imaging imaging microscope. Journal of Biomedical Optics, 19:4 046010-1 – 046010-10 (2014)
  6. Peter F. Favreau, Clarissa Hernandez, Ashley Stringfellow-Lindsey, Diego F. Alvarez, Thomas C. Rich, Prashant Prabhat, Silas J. Leavesley. Tunable thin-film optical filters for hyperspectral microscopy. Journal of Biomedical Optics, 19:1 011017-1 – 011017-11 (2014)
  7. Thomas C. Rich, Kristal Webb, Silas J. Leavesley. Perspectives on Cyclic Nucleotide Microdomains and Signaling Specificity: Can we decipher the information content contained within cyclic nucleotide signals? Journal of General Physiology, 143:17-27 (2014), PMCID: PM3874573


  1. Silas J. Leavesley, Andrea Britain, Lauren Cichon, Thomas C. Rich. Assessing FRET using spectral techniques. Cytometry, Part A, 83:10 898-912 (2013)
  2. Naga S. Annamdevula, Brenner Sweat, Peter Favreau, Ashley S. Lindsey , Diego F. Alvarez , Thomas C. Rich, Silas J. Leavesley. An approach for characterizing and comparing hyperspectral microscopy systems. Sensors 13:7 9267-9293 (2013), PMCID: PMC375864
  3. Birsen Sirkeci, Mallika Sridhar-Keralapura, Serena Coelho, Silas Leavesley, Thomas C. Rich. Linear unmixing of hyperspectral images for analysis of fluorescently-labeled cells with imperfect endmember spectra. Proc. ISBI 683 (2013)


  1. Francis M, Qian X, Charbel C, Ledoux J, Parker JC, Taylor MS. Automated region of interest analysis of dynamic Ca2+ signals in image sequences. Am J Physiol Cell Physiol. 303(3):C236-43 (2012)
  2. Taylor MS, Francis M, Qian X, Solodushko V. Dynamic Ca(2+) signal modalities in the vascular endothelium. Microcirculation. 19(5):423-9 (2012)
  3. Silas J. Leavesley, Naga Annamdevula, John Boni, Samantha Stocker, Kristin Grant, Boris Troyanovsky, Thomas C. Rich, Diego F. Alvarez. Hyperspectral imaging microscopy for identification and quantitative analysis of fluorescently labeled cells in highly autofluorescent tissue. Journal of Biophotonics 5:1 67-84 (2012), PMCID: PM3517021
  4. Wei P. Feinstein, Bing Zhu, Silas J. Leavesley, Sarah L. Saynor, Thomas C. Rich. Assessment of cellular mechanisms contributing to cAMP compartmentalization in pulmonary microvascular endothelial cells. American Journal of Physiology – Cell Physiology 302:6 C839-C852 (2012), PMCID: PM3311237